吸附剂及培养基组成对香荚兰细胞悬浮培养产生香兰素的影响  

EFFECTS OF ABSORBENTS AND COMPOENTS OF MEDIUM ON VANILLIN BIOSYSTHESIS IN CELL CUTURES OF VANILLA PLANIFOLIA

作　　者：曹孟德[1] 李家儒[2] 秦东春 王君健[3] 

机构地区：[1]郑州大学医学院,郑州450052 [2]武汉大学生命科学院,武汉430072 [3]华中理工大学生物工程系,武汉430074

出　　处：《植物研究》2002年第1期65-67,共3页Bulletin of Botanical Research

摘　　要：本文报道了培养基的组成及添加吸附剂对香荚兰细胞悬浮培养产生香兰素的影响。结果表明 ,添加吸附剂活性炭及XAD - 2后 ,香荚兰细胞产生的香兰素含量明显增加 ,而且活性炭的效果优于XAD - 2 ;香荚兰细胞在全组成的MS培养基中香兰素含量均低于由矿物质盐组成培养基中的含量。可以考虑采用二步培养法来培养香荚兰细胞及产生香兰素。This paper reported the effects of absorbents and composition of medium on vanillin biosynthesis in cell cultures of Vanilla planifonia. It was shown that the absorbents XAD-2 and activated charcoal had a marked increasing effect on the accumulation of vanillin, and the activated charcoal had more marked increasing effect than XAD-2;and that the contents of vanillin of Vanilla planifonia cells cultured on the complete MS medium were lower than that on the medium composed of mineral salts .It was suggested that two-step culture should be taken during the production of vanillin of Vanilla planifonia cells in batch suspension culture.

关 键 词：香英兰 香兰素 吸附剂 细胞悬浮培养 培养基 香料 

分 类 号：S573.9[农业科学—作物学]