一个新的人乳源性生物活性肽cDNA克隆与测序分析  

THE CLONING AND SEQUENCING OF A PROLACTIN -LIKE cDNA IN HUMAN MILK

作　　者：董克家[1] 

机构地区：[1]海南医学院转基因实验室,海口571101

出　　处：《海南医学院学报》2001年第4期193-194,共2页Journal of Hainan Medical University

基　　金：高等学校骨干教师资助(2000-65);海南省百项农业新技术基金资助(1998-149)

摘　　要：目的:分离、克隆人乳汁中新的生物活性肽。方法:从人初乳中提取总RNA,反转录获得cDNA,SMARTPCR扩增cDNA片段,克隆于pGEM-TEasy载体中,进行测序分析。结果:克隆一个新的cDNA并编码42个氨基酸的多肽,与人催乳素样糖蛋白部分同源,加入国际基因库,编号为:GenbankAF160979。结论:人初乳中存在核酸RNA,所克隆的新cDNA可能具有催乳素样功能。Objective:To isolate and clone a new cDNA in human milk.Method:The milk RNA was pu rified and re versed to cDNA.SMART PCR was used to construct the Library.Result:By DNA se quencing,a new cDNA en coding a predicted peptide of42residues was revealed,which shared41.5%amino acid and identified with prolactin-Like pro tein.Conclu sion:These data suggest that the predicted pep tide may play important roles in reproductive physiology.

关 键 词：基因 序列分析 乳 人 生物活性肽 CDNA 克隆 

分 类 号：R394.8[医药卫生—医学遗传学] Q785[医药卫生—基础医学]