蝎毒抗癌多肽对人肝癌细胞株的作用  

The effect of APBMV on human hepotoma cell line

作　　者：魏玲[1] 孔天翰[2] 董伟华[2] 

机构地区：[1]山东省肿瘤防治研究中心实验室,济南250117 [2]河南医科大学生物毒素中心

出　　处：《肿瘤防治研究》2002年第1期9-11,共3页Cancer Research on Prevention and Treatment

基　　金：河南省"九五"科技攻关项目(951200102)

摘　　要：目的 观察蝎毒抗癌多肽（ＡＰＢＭＶ）对人肝癌细胞ＳＭＭＣ－７７２１的作用。方法ＭＴＴ法、生长抑制实验和集落形成实验。结果 ＡＰＢＭＶ对 ＳＭＭＣ－７７２１细胞具较强毒性作用，ＩＣ５０为１１．３３μｇ／ｍｌ；ＡＰＢＭＶ可显著抑制细胞生长，剂量与时间效应关系明显。结论ＡＰＢＭＶ是东亚钳蝎蝎毒中有效低毒的抗肿瘤成分，对肝癌细胞有一定的毒性作用。Objective To obserne the effect of antineoplastic polypeptide from Buthus martensii venom (APBMV) on the human heptoma cells SMMC-7721. Methods Using the MTT colorimetric method, the growth-inhibiting test and the colony-formation inhibiting assay. Results The results revealed that APBMV had obvious cytotoxicity on SMMC-7721,1C50 was 11. 33μg/ml;APBMV could obviously inhibit the cellu- lar growth. There were obvious dose-response and timeresponse relationship. The IC50 were 15. 8T54ig/ml, 13. 05μg/ml,8. 70μg/ml,respectively at 24h,48h and 96h intervals after SMMC-7721 cells were exposed to APBMV. There were instinct correlations in inhibiting effect between24h and 48h, or 48h and 96h(rrr9709,P<0. 05;r0. 9831,P<0. 02). When the concentration of APBMV exceeded 8μg/ml,the colony- formation numbers of cells reduced dramatically(P<0. 01) ,1C50 was 10. 83μg/ml. Conclusion APBMV was effective and low toxicity antineoplastic component in the venom of Buthus Martensii scorpiton. It posses certain toxicity on hepatoma cells.

关 键 词：蝎毒 肝癌 细胞株 多肽 

分 类 号：R735.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]