整合在鼻咽癌细胞株SUNE-1中的EB病毒LMP-1基因的缺失性改变  

Deletion of the LMP-1 gene integrated in the nasopharyngeal carcinoma cell line SUNE-1

作　　者：刘鹏[1] 朱振宇[1] 张清秀[1] 黄必军[2] 李满枝[2] 黄丹云[1] 马涧泉[1] 

机构地区：[1]中山医科大学生物化学教研室,广东广州510089 [2]中山医科大学肿瘤研究所,广东广州510089

出　　处：《中国病理生理杂志》2001年第8期722-725,共4页Chinese Journal of Pathophysiology

基　　金：国家教育部博士点基金资助项目 (No .97- 95 );广东省自然科学基金资助项目 (No 9940 44 )

摘　　要：目的 :研究EBVLMP - 1基因在SUNE - 1细胞染色体上的整合情况。方法 :采用PCR方法检测整合于SUNE - 1染色体的LMP - 1基因 ;限制性内切酶酶切和PCR方法初步验证部分LMP - 1基因发生缺失 ;DNA测序进一步确定LMP - 1基因的缺失情况。结果 :LMP - 1基因在SUNE - 1细胞染色体上的整合有完整与缺失两种情况 ;其缺失发生于其两个内含子部分。结论 :整合于SUNE - 1染色体DNA上的EBVLMP - 1,有部分发生了内含子 1与AIM: To study the EBV LMP-1 gene integrated in the chromosome of poorly differentiated nasopharyngeal carcinoma cell line SUNE-1. METHODS: The LMP-1 gene of SUNE-1 was detected with PCR; Deletion of LMP-1 was examined by restriction endonuclease analysis and PCR. The deletion was precisely localized by DNA sequencing. RESULTS: The LMP-1 gene integrated in the chromosome of SUNE-1 could be deleted or non-deleted. The two introns of LMP-1 gene were shown being lost in SUNE-1 cell line. CONCLUSION: Deletion of intron 1 and intron 2 happen in some of the LMP-1 gene integrated in the chromosome of SUNE-1.

关 键 词：爱泼斯坦-巴尔病毒 染色体缺失 鼻咽肿瘤 鼻咽癌 整合 EBV 

分 类 号：R739.63[医药卫生—肿瘤]