人前列腺癌相关基因pc-1表达产物在细胞内的定位研究  

Localization of the expression product of human prostatic cancer associated pc-1 gene in cells

作　　者：张浩[1] 周建光[1] 孙玉龙[1] 李杰之[1] 袁兰 黄翠芬[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850 [2]北京大学医学部医药卫生分析中心,北京100083

出　　处：《军事医学科学院院刊》2001年第4期255-259,264,共6页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助项目 ( 30 0 70 2 96 )

摘　　要：目的 :研究pc 1基因表达产物在细胞内的定位。方法 :采用巢式PCR技术 ,从原始克隆质粒中扩增出可编码蛋白分子的pc 1cDNA片段 ,分别将其克隆入含EGFP和MYC标签的真核表达载体中 ;用脂质体介导法将其转染入人前列腺癌细胞C4 2中 ;通过免疫细胞化学方法和激光共聚焦显微镜技术检测pc 1表达产物在细胞内的定位。结果与结论 :pc 1表达产物定位于细胞质中 ,为进一步研究其生理、生化功能奠定了基础。Objective:To investigate the localization of the expression product of pc 1 gene in cells. Methods: The protein coding region of pc 1 cDNA was amplified using nested PCR technology from the primary cloning plasmid and subcloned into the eukaryotic expressing vectors pEGFP N1 and pCDNA3.1( )/myc his containing EGFP and MYC tag,respectively,then transfected into the human prostatic cancer cell line C4 2.The location of its expression product in C4 2 was observed with immunocytochemistry and laser confocal scanning microscopy. Results and Conclusions:The expression product of pc 1 gene located in the cytoplasm. This work provides the basis for further study of the biochemical and physiological functions of the pc 1 gene.

关 键 词：前列腺肿瘤 pc-l 免疫细胞化学 激光共聚焦 细胞定位 

分 类 号：R737.25[医药卫生—肿瘤]