用于测量农药残留的小麦酯酶的选择  

Selection of Plant-esterase for Detection of Pesticide Residue

作　　者：肖建军[1] 华泽钊[1] 徐斐[1] 李保国[1] 

机构地区：[1]上海理工大学食品科学与工程研究所,上海200093

出　　处：《分析测试学报》2002年第2期11-14,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金重点项目 (59836240)

摘　　要：为研制探测农药残留的生物传感器 ,研究了农药乐果[O,O_二甲基S_(N_甲基胺基甲酰甲基 )二硫代磷酸酯]对各种小麦植物酯酶的抑制 ;从小麦中提取植物酶 ,以农药乐果为抑制剂 ,采用分光光度法研究了乐果对各种小麦酯酶活性的影响 ;研究显示 ,不同品种小麦酯酶对农药乐果的敏感度不同 ,在所研究的品种中 ,豫麦39和小麦周9对乐果较敏感 。The inhibition effect of pesticide of dimethoate on various plant _ esterases has been studied to develop a biosensor for detection of pesticide residue. Effect of the inhibitor of dimethoate on activities of plant _ esterases from different kinds of wheats was evaluated by spectrophotometric measurement. The results indicated the inhibiting sensitivity of dimethoate to plant _ esterases from different wheats were not the same. Among the kinds of wheate studied, the esterases from the wheats of Yumai 39 and Zhou 9 are more sensitive than others. It was found that the selection of wheat plant _ esterases for cheap detection of pesticide residue is necessary.

关 键 词：农药残留 生物传感器 植物酶 小麦酯酶 有机磷农药 乐果 检测 农产品 酶活性 

分 类 号：TS207.53[轻工技术与工程—食品科学] S481.8[轻工技术与工程—食品科学与工程]