吸附—交联法固定胰蛋白酶及在澄清啤酒中的应用  

Study on Immobilization of Trypsin by Absorbing and Crosslinking and Its Application in Beer Clarification

作　　者：温燕梅[1] 邱彩虹[1] 

机构地区：[1]湛江海洋大学基础科学系,广东湛江524088

出　　处：《湛江海洋大学学报》2001年第4期42-46,共5页Journal of Zhanjiang Ocean University

摘　　要：胰蛋白酶的固定化采用吸附—交联法。在单因素试验基础上 ,通过正交试验 ,获得了胰蛋白酶固定化的最佳条件 :酶与载体体积比为 2∶ 1,吸附时间为 10 h,p H=4 .5 ,缓冲液浓度为 0 .0 5 mol/ L,戊二醛的质量分数为 0 .1%。此条件下制得的磁性酶活力回收在 5 0 %左右 ,相对酶活力达 74 % 。Trypsin is immobilized on magnetic colloid through absorbing and crosslinking. On the basis of single factor experiments,the best condition of the immobilization of trypsin can be found through orthogornal experiments:The ration of enzyme to carriers is two to one ,absorption time 10 hours,pH=4.5,concentration of the buffer solution 0.05 mol/L,quality fraction of the glutaril dialdehyde, 0.1%.The activity recovery of immobilized enzyme is around 50% and relative enzyme activity 74% under these conditions. MIE can clarify beer and prevent cold turbid.

关 键 词：胰蛋白酶 固定化 磁性酶 啤酒 澄清 吸附-交联法 

分 类 号：TS262.5[轻工技术与工程—发酵工程] Q556.3[轻工技术与工程—食品科学与工程]