分光光度法测定血液制品生产过程中的乙醇含量  

Determination of ethanol content by spertrophotometry in the processing of blood preparation

作　　者：何彦林 周耀东[2] 杨晓东[2] 张璘[2] 韩国德[2] 赵祖南[2] 魏国宏[2] 

机构地区：[1]甘肃工业大学石油化工学院,730050 [2]兰州生物制品研究所

出　　处：《兰州医学院学报》2002年第1期9-11,共3页Journal of Lanzhou Medical College

摘　　要：目的　建立血液制品生产过程中乙醇含量的测定方法。方法　在酸性条件下曙红与乙醇反应显橙色 ,被疏水有机溶剂提取后 ,用分光光度法测定乙醇含量。结果　在 480nm处有机相吸收值与乙醇含量 (≤ 45 % )呈正切函数关系。结论　用该法对血液制品生产过程中各组分悬浊液和上清液中乙醇含量进行了测定。结果表明 ,该法具有快速、准确。Objective To determinate the method for detection ethanol content in the processing of blood `preparation.Methods Eosin Y and ethanol reacted on acid condition to produce an orange compound which can be extracted by hydrophobic organic solvent and the ethanol content can be detected with spertrophotometry.Results There is tangent function relation between organic solvent optical density and ethanol concentration(≤45%).Conclusion The method was used to determinate ethanol content in every suspension and supernatant in the processing of blood preparation, the result showed the method is fast, accurate, convenient and a better repeaty.

关 键 词：分光光度法 血液制品 乙醇 含量测定 低温乙醇法 

分 类 号：R457.1[医药卫生—治疗学] TQ464.5[医药卫生—临床医学]