D-泛解酸内酯水解酶产生菌的筛选及产酶条件研究  

Production of D-pantolactone Hydrolase by Fusarium moniliforme SW-902

作　　者：汤一新 孙志浩[1] 华蕾[1] 过鑫富 汪军 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214036 [2]浙江鑫富生化股份有限公司,杭州311301

出　　处：《微生物学报》2002年第1期81-87,共7页Acta Microbiologica Sinica

基　　金：国家十五科技攻关项目部分内容 ( 2 0 0 1BA70 8B5 )

摘　　要：筛选到一株产D 泛解酸内酯水解酶的菌株 ,经鉴定为串珠镶孢霉菌 (Fusariummonili forme)SW 90 2。产酶条件研究表明 ,用甘油作碳源 ,蛋白胨作氮源 ,初始pH8 0 ,温度 2 6℃ ,摇瓶培养 3d ,产酶量最高。在 6 0L发酵罐中通风发酵 45h ,产菌丝体生物量 7 1 8g干菌体 L ,D 泛解酸内酯水解酶酶活力达到 0 92IUFusarium moniliforme SW-902, a high-yielding strain for producing D-pantolactone hydrolase was screened. The conditions for enzyme formation of the strain were studied. The suitable carbon source of the medium was glycerol and the suitable nitrogen source was peptone. The optimum temperature and initial pH for enzyme formation were 26℃ and pH8 0 respectively. When the organism was cultured in the 60L-fermentor under the optimum conditions for 45h, about 7.18g dry cells/L and 0.92 IU/g dry cells weight were obtained.

关 键 词：D-泛解酸内酯水解酶 串珠镰孢霉菌SW-902 D-泛酸 菌株筛选 产酶条件 

分 类 号：TQ920[轻工技术与工程—发酵工程]