Ki-Ras反义寡核苷酸对人胃癌细胞及血管内皮细胞的作用  

Effect of antisense oligodeoxynucelotide specific to ki-Ras gene on human gastric carcinoma cells and human vascular endothelial cells

作　　者：任娟[1] 董蕾[2] 徐仓宝[3] 李明众[1] 王晖[3] 王晓丽[1] 王梅[3] 

机构地区：[1]西安交通大学第一医院肿瘤放疗科,西安710061 [2]西安交通大学第二医院消化内科,西安710004 [3]西安交通大学医学院,西安710061

出　　处：《西安医科大学学报》2002年第1期30-34,共5页Journal of Xi'an Medical University(Chinese)

基　　金：:陕西省科委科技攻关项目 ( 2 0 0 0 KX6 15 )

摘　　要：:目的　用Ki Ras的反义寡核苷酸 (ASODN)作用于人胃癌细胞和血管内皮细胞 ,为其抑制胃癌生长及其内血管生成进行前期基础研究。方法　MTT法观察Ki RasASODN对两种细胞增殖的影响 ,电镜下观察细胞的超微结构变化及有无凋亡发生。结果　Ki RasASODN可明显抑制人胃癌细胞和血管内皮细胞的增殖。在一定剂量下还可诱发细胞凋亡。结论　Ki RasP2 1在人胃癌细胞和血管内皮细胞的增殖与凋亡调控中具有重要作用。Objective To study the effect of Ki Ras ASODN on human gastric carcinoma cells and human vascular endothelial cells as a preclinical experimental research of the inhibition of tumor development and angiogenesis by Ras ASODN. Methods The proliferation of HGCC and HVEC was determined by MTT method. The ultrostructural change was observed under electron microscope. Results Ki Ras ASODN inhibited the growth of human gastric carcinoma cells and human vascular endothelial cells remarkably. A certain amount of dose induced apoptosis.Conclusion Ras P21 plays a key role in regulating the proliferation and apoptosis of HGCC and HVEC.

关 键 词：KI-RAS 反义寡核苷酸 胃癌细胞 血管内皮细胞 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤] R730.2[医药卫生—临床医学]