HTLV-1转录激活因子Tax和Taxreb107的相互作用  

Interaction between HTLV-1 Transcription Activator Tax and Taxreb107

作　　者：杨曦[1] 王冀姝[1] 韩骅[1] 

机构地区：[1]第四军医大学医学遗传学与发育生物学教研室,西安710032

出　　处：《生物化学与生物物理学报》2002年第2期231-235,共5页

基　　金：国家自然科学基金资助项目 (No .39970 376 )~~

摘　　要：Tax是人类T淋巴细胞白血病病毒编码的转录因子。核糖体蛋白RpL6又称Taxreb10 7,是Tax应答序列结合蛋白 ,二者均作用于HTLV 1的启动子LTR。用酵母双杂交法和GST下拉检测 (pull down)法研究了Tax和Taxreb10 7/RpL6之间的相互关系。结果显示 ,二者在酵母细胞内和体外均具有直接相互作用。这些结果提示Taxreb10Tax is a transcription activator encoded by human T-cell leukemia virus (HTLV)-1. Ribosomal protein L6 was also defined as Taxreb107 (Tax responsible element binding protein 107) for its activity of binding to the long terminal repeats of HTLV-1. To investigate the relationship between Tax and Taxreb107/RpL6, yeast two hybrid and GST pull-down assays were used. Results suggest that Tax can interact with Taxreb107/RpL6 directly and Taxreb107/RpL6 may regulate the function of Tax in HTLV-1 proliferation.

关 键 词：TAX Taxreb107/RpL6 HTLV-1 酵母双杂交 转录激活因子 相互作用 白血病毒 

分 类 号：Q78[生物学—分子生物学]