Establishment of 6VS Telocentric Lines of Haynaldia villosa Resistant to Powdery Mildew Induced by Immature Embryo Culture  

幼胚培养创造抗白粉病簇毛麦6VS端体(英文)

作　　者：李辉[1] 陈孝[1] 辛志勇[1] 徐惠君[1] 杜丽璞[1] 马有志[1] 

机构地区：[1]中国农业科学院作物育种栽培研究所农业部作物遗传育种重点实验室

出　　处：《Acta Botanica Sinica》2002年第2期127-131,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家九.五科技攻关项(85-002-02-03)~~

摘　　要：The line of T240-6 was selected from 32 SC 2 lines of immature embryo culture of T240 (common wheat (Triticum aestivum L.)× Wheat-Haynaldia villosa (L.) Schur. 6D/6V substitution line) through powdery mildew characterization, glutamate oxaloacetate transaminase (GOT) enzyme and gliadin (Gli) analyses and in situ hybridization. All of the individual plants resistant to powdery mildew lacked the locus of GOT at 6VL arm (GOT-V 2) and had gliadin locus at 6VS arm (Gli-V 2) of Haynaldia villosa. All the plants resistant to powdery mildew had one or two telocentric chromosomes that did not pair with wheat chromosomes but paired between themselves. T240-6 was identified as a telocentric line through in situ hybridization.通过白粉病抗性鉴定、生化标记及分子原位杂交相结合的方法 ,从小麦 (TriticumaestivumL .)幼胚培养组合T2 40 (普通小麦×小麦_簇毛麦 (HaynaldiavillosaLam .) 6D/6V异代换系 )的 32个SC2 代株系中 ,筛选出T2 40_6株系 ,其所有的抗白粉病单株均缺失簇毛麦 6V染色体长臂上的谷草转氨酶位点GOT_V2 ,而具有短臂上的醇溶蛋白位点Gli_V2 。细胞学染色体观察表明 ,该株系的所有抗病单株均具有 1～ 2个端体 ,这些端体不能与小麦染色体配对 ,双端体之间可以配对。经原位杂交分析 ,端体杂交呈阳性 ,表明它们均为簇毛麦 6V染色体短臂 (6VS)。

关 键 词：Haynaldia villosa immature embryo culture telocentric chromosome glutamate oxaloacetate transaminase GLIADIN in situ hybridization 

分 类 号：S512.903.53[农业科学—作物学]