氟胞嘧啶/胞嘧啶脱氨酶自杀基因疗法结合热休克蛋白多肽复合物治疗小鼠胃癌  

Therapy of murine gastric cancer by 5-FC/CD suicide gene system combined with HSP-PC tumor vaccine

作　　者：陈平[1] 陈志琳[2] 徐立春[2] 孙振华[2] 施诚仁[3] 

机构地区：[1]苏北人民医院外科,江苏扬州225001 [2]扬州大学医学院肿瘤免疫研究中心,江苏扬州225001 [3]上海第二医科大学附属新华医院,上海200092

出　　处：《中国癌症杂志》2002年第1期31-34,共4页China Oncology

基　　金：江苏省社会发展基金项目 (No .BS99381)

摘　　要：目的 :研究氟胞嘧啶 /胞嘧啶脱氨酶 (5 FC/CD)基因疗法与热休克蛋白 多肽复合物 (HSP70 PC)免疫疗法联合抗肿瘤效果。方法 :将携带CD基因的重组腺病毒注射到小鼠MFC瘤体内 ,腹腔注射 5 FC ,同时皮下接种HSP70 PC。结果 :经联合治疗后 ,70 %荷瘤小鼠肿瘤体积缩小 ,消退 ,小鼠存活期延长 ,细胞毒T淋巴细胞(CTL)杀伤活性增高 ,CD+ 4及CD+ 8T细胞浸润明显。结论 :5 FC/CD基因疗法结合HSP PC免疫疗法抗小鼠MFC瘤作用显著 ,具有临床应用前景。Purpose:To investigate the antitumor effect of 5-fluorocytosine/cytosine deaminase (5FC/CD) suicide gene system combined with heat shock protein 70 peptide complexes(HSP70-PC) tumor vaccine.Methods:5-FC was administrated intraperitoneally after recombinant adenoviruses carrying E.coli CD gene were injected into murine tumor tissue. At the same time,mice bearing tumor were inoculated s.c. with HSP70-PC. Results:The combined therapy led to reduction in tumor growth, and complete tumor regression was observed in 70% of the mice. After the combined therapy, the tumor tissue showed more CD + 4 and CD + 8T cells infiltration, and the murine CTL activities increased significantly. Conclusions:The therapy combining 5-FC/CD system with HSP-PC tumor vaccine is very effective in murine gastric cancer, and has potential for clinical usage.

关 键 词：自杀基因 热休克蛋白-多肽复合物 MFC胃癌细胞 氟胞嘧啶/胸嘧啶脱氨酶 小鼠 

分 类 号：R735.2[医药卫生—肿瘤]