大孔树脂纯化红树莓籽黄酮及其体外结合胆酸盐能力评价  

Purification of Flavonoids from Red Raspberry Seed by Macroporous Resin and the Ability of Bile Salt-binding in Vitro

作　　者：芦宇 纪秀凤 吕长鑫 常俏 王圆圆 于静雯 王维民 王贵虹 励建荣 LU Yu;JI Xiu-feng;LV Chang-xin;CHANG Qiao;WANG Yuan-yuan;YU Jing-wen;WANG Wei-min;WANG Gui-hong;LI Jian-rong(College of Food Science and Technology,Bohai University,Food Safety Key Lab of Liaoning Province,National &Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricuhural and Aquatic Products,Jinzhou 121013,China;Dalian Zhongchao Food Co.,Ltd.,Dalian 116400,China;Liaoning Xindadi Industrial Development Group Co.,Ltd.,Shenyang 110168,China)

机构地区：[1]渤海大学食品科学与工程学院,辽宁省食品安全重点实验室,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013 [2]大连中超食品有限公司,辽宁大连116400 [3]辽宁新大地实业发展集团有限公司,辽宁沈阳110168

出　　处：《食品工业科技》2018年第23期7-11,共5页Science and Technology of Food Industry

基　　金：国家重点研发计划专项(2017YFD0400704、2016YFD0400805).

摘　　要：以红树莓籽黄酮为研究对象,考察了AB-8大孔树脂纯化黄酮最佳条件,并研究不同提取方法和纯化对产品胆酸盐结合能力的影响。结果表明:红树莓籽黄酮上样浓度10 mg/m L,AB-8大孔树脂最佳纯化条件为:上样量100 m L、pH4.5、上样流速1.5 m L/min,以60%乙醇、150 m L、流速1.5 m L/min洗脱,红树莓籽黄酮纯度可达48.19%,与未纯化相比提高了23.12%;醇提、超声波、酶提和AB-8纯化4种方式所得黄酮,对3种胆酸盐结合能力依次为:AB-8纯化、醇提、超声波和酶提黄酮,其中AB-8纯化黄酮对牛磺胆酸钠、甘氨胆酸钠、胆酸钠3种胆酸盐结合能力依次为79.35%、74.88%和74.56%,结果表明,红树莓籽黄酮对胆酸盐均有一定结合能力,其中AB-8纯化黄酮结合能力最强。The purification conditions of red raspberry seed flavonoids by AB-8 macroporous resin were investigated and its ability of binding bile salts was evaluated.The optimal purification conditions of AB-8 macroporous resin was as follows: Sample concentration was 10 mg/m L,sample volume was 100 m L,the sample flow rate was 1.5 m L/min and sample p H was 4.5,the volume fraction of the ethanol was 60%,the volume of elution was 150 m L,and the flow rate of elution was 1.5 m L/min.The purity of red raspberry seed flavonoids was 48.19% and which was 23.12% higher than those unpurified. And four kinds of extraction methods for the three kinds of bile salt binding ability was in the order: purified by AB-8,ethanol extracted,ultrasonic extracted and enzyme extracted flavonoids. Moreover,hypolipidemic experiments showed that purified flavonoids by AB-8 had strong combining capabilities for sodium taurocholate,sodium glycocholate,sodium cholate with combination rate of79.35%,74.88% and 74.56%. The results showed that the red raspberry seeds flavonoids had ability to bind to bile salts,among which AB-8 had the strongest binding ability.

关 键 词：红树莓籽 黄酮 大孔树脂 纯化 胆酸盐 降血脂 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]