常见食用肉中狐源性成分DNA检测试剂盒的研制与应用  

Development on DNA detection kit of fox and its application in common meat products

作　　者：艾金霞 惠赫童 高丽君 孙丽媛 李明成 夏薇 苑广信[2] 张丽华 段思琪 李盈诺 陈堃 Ai Jinxia;Hui Hetong;Gao Lijun;Sun Liyuan;Li Mingcheng;Xia Wei;Yuan Guangxin;Zhang Lihua;Duan Siqi;Li Yingnuo;Chen Kun(School of Medical Technology,Beihua University,Jilin 132013,China)

机构地区：[1]北华大学医学技术学院,吉林132013 [2]北华大学药学院 [3]吉林市雷博科技有限公司

出　　处：《卫生研究》2018年第6期979-983,共5页Journal of Hygiene Research

基　　金：吉林省科技发展计划(No.20160204004NY;20160307030YY;20170307001YY);吉林市科技创新发展计划(No.20162006);吉林省大学生创新创业训练计划(No.199117138-2017)

摘　　要：目的研制狐源性成分DNA提取和检测试剂盒。方法对经典DNA提取方法进行改良,研制了狐源性成分DNA提取和检测试剂,开发了狐源性成分DNA检测试剂盒,并对试剂盒检测参数进行评价。应用该试剂盒对狐肉与42种市售食用肉制品混合样品进行检验。结果狐肉DNA检测试剂盒反复冻融处理5、10和20次后依然有效,可于-20℃保存1年。狐肉与42种常见食用肉类混合样品均可检出狐源性成分,试剂盒特异性为100%;样品DNA最低检测限为0. 1 ng/μL。结论狐源性成分DNA检测试剂盒适用于常见食用肉中狐源性成分快速检测,且特异性好、灵敏度高、稳定性强。Objective To develop a kind of DNA extraction and detection kit for identification of fox source composition.Methods Using the modern DNA fingerprint technology,the DNA extraction method was improved,and DNA extraction and detection reagent was developed to obtain the fox polymerase chain reaction (PCR) detection kit.The performance parameters of the kit were evaluated.Finally,42 samples of fox meat and its mixture with commercial meat products were detected.Results The kit was proved effective after 20 times of the repeated frozen-thaw and it could be stored at -20℃ for 1year.The specificity test confirmed that fox source composition were detected from 42samples of fox meat and its mixture with commercial meat.The specificity of the kit was 100%.The minimum detection limit of DNA was 0.1ng/μL. Conclusion The fox DNA detection kit could be applied in rapid detection common meat of fox source composition,which are good specificity,high sensitivity and good stability.

关 键 词：狐源性成分 聚合酶链式反应 DNA检测试剂盒 常见食用肉 食品安全 

分 类 号：R155.55[医药卫生—营养与食品卫生学]