Inhibitory Effect of LPS on the Proliferation of Oligodendrocyte Precursor Cells through the Notch Signaling Pathway inIntrauterine Infection-induced Rats  

作　　者：Yan-qin YING Xue-qin YAN Sheng-juan JIN Yan LIANG Ling HOU Wan-ting NIU Xiao-ping LUO 

机构地区：[1]Department of Pediatrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [2]Department of Children Healthcare,Zhongshan Boai Hospital Affiliated to Southern Medical University,Zhongshan 528403,China [3]VA Boston Healthcare System,Department of Orthopedics,Brigham and Women's Hospital,Harvard Medical School,Boston 02130,USA

出　　处：《Current Medical Science》2018年第5期840-846,共7页当代医学科学（英文）

基　　金：This project was supported by grants from Natural Science Foundation of China,Hubei Province (No.2017CFB645)and National Natural Science Foundation of China (No.81471519).

摘　　要：Periventricular white matter injury (PWMI)is very common in survivors of premature birth,and the final outcomes are a reduction in myelinated neurons leading to white matter hypomyelination.How and (or) why the oligodendrocyte lineage develops abnormally and myelination is reduced is a hot topic in the field.This study focuses on the effect of intrauterine inflammation on the proliferation of oligodendrocyte lineage cells and the underlying mechanisms.Lipopolysaccharide (LPS)(300μg/kg)was intraperitoneally injected into pregnant Sprague-Dawley rats at embryonic days 19 and 20 to establish a rat model of intrauterine infection-induced white matter injury.Corpus callosum tissues were collected at postnatal day 14(P14)to quantify the number of oligodendrocytes,the number and proliferation of oligodendrocyte precursor cells (OPCs), and the expression of myelin proteins (MBP and PLP).Furthermore,the expression of Writ and Notch signaling-related proteins was analyzed.The results showed that the number of oligodendrocytes in the corpus callosum tissues of LPS-treated rats was reduced,and the expression levels of myelinating proteins were down-regulated.Further analysis showed that the Notch signaling pathway was down-regulated in the LPS-treated group.These results indicate that intrauterine LPS may inhibit the proliferation of OPCs by down-regulating the Notch rather than the Writ signaling pathway,leading to hypomyelination of white matter.

关 键 词：OLIGODENDROCYTE PRECURSOR cells INTRAUTERINE infection HYPOMYELINATION LIPOPOLYSACCHARIDE signaling pathway 

分 类 号：R[医药卫生]