食用植物油中3-MCPD酯GC-MS测定方法研究  

Determination of 3-MCPD Esters in Edible Vegetable Oil by GC-MS

作　　者：王永瑞[1,2] 王丹 徐宇丽 魏长庆 Wang Yongrui;Wang Dan;Xu Yuli;Wei Changqing(Food College,Shihezi University,Shihezi 832000;Key Laboratory of Xinjiang Plant Medicine Resource Utilization,Shihezi 832000;Shihezi Entry -Exit Inspection and Quarantine Bureau,Shihezi 832000)

机构地区：[1]石河子大学食品学院,石河子832000 [2]新疆植物药资源利用教育部重点实验室,石河子832000 [3]石河子出入境检验检疫局,石河子832000

出　　处：《中国粮油学报》2018年第11期99-104,共6页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金(31401617);国家自然科学基金(31360389;31760434);中国博士后科学基金第59批面上资助项目(168667);石河子大学高层次人才项目(RCZX201524)

摘　　要：建立了甲醇钠-甲醇水解,固相萃取柱净化,HFBI衍生结合气相色谱-质谱(GC-MS)方法对食用植物油中3-MCPD酯含量进行测定。该方法在0.50～2. 50 mg/L范围内线性关系良好(r^2=0.988 5),检出限为0. 025 5 mg/kg,定量限为0. 093 3 mg/kg。3-MCPD酯的平均加标回收率在94. 80%～104. 62%之间,RSD 1.47%～5.23%。本方法操作简单、灵敏度高、重复性好,能够很好地适用于食用植物油中3-MCPD酯的检测。采用该方法对新疆几种市售食用植物油中的3-MCPD酯进行测定。新疆市售的几种食用植物油中均检测出浓度不等的3-MCPD酯。其中菜籽油、亚麻籽油、番茄籽油、花生油以及葡萄籽油受3-MCPD酯污染严重,3-MCPD酯含量都高于0.4 mg/kg。而核桃油、大豆油、红花籽油中3-MCPD酯的含量都低于0.4mg/kg。A method,hydrolyzed by sodium methoxide -methanol,purified by solid phase extraction (SPE) using diatomaceous earth as the sorbent,derivatized with heptafluorobutyrylimidazole (HFBI)and combined with gas chromatography-mass spectrometry (GC -MS)was established in order to detecting the content of 3-MCPD ester in edible plant oils.The method showed good linear relationship in the range of 0.50-2.50mg/kg (r2=0.9885). The detection limit and quantification limit were 0.0255mg/kg and 0.0933mg/kg respectively.The recovery rate was between 94.80%-104.62%.and the relative standard deviation was between 1.47%-5.23%.The method was easily operated with good sensitivity and repeatability,and it could meet the determination demand of 3-MCPD ester in edible plant oil.The content of 3-MCPD ester in several edible plant oil of Xinjiang was detected,and the results shown :3-MCPD ester was found in all of edible plant oil with different concentration.Rapeseed oil,flaxseed oil,tomato seed oil,peanut oil,and grape seed oil were severely contaminated with 3-MCPD esters,and the content of 3-MCPD esters were both higher than 0.4 mg/kg. and safflower oil were both lower than 0.4 mg/kg. The content of 3-MCPD esters in walnut oil,soybean oil

关 键 词：食用植物油 3-MCPD酯 气相色谱-质谱(GC-MS) 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]