葡萄状枝瑚菌菌丝液体培养条件优化  

Optimization of Liquid Culture Conditions of Mycelium of Ramaria botrytis

作　　者：余炫彤 陈双林[1] 何刚[1] 林羲[1] 闫淑珍[1] YU Xuan-tong;CHEN Shuang-lin;HE Gang;LIN Xi;YAN Shu-zhen(College of Life Sciences ,Nanjing Normal University,Nanjing 210023,China)

机构地区：[1]南京师范大学生命科学学院,江苏南京210023

出　　处：《食品工业科技》2018年第24期102-108,共7页Science and Technology of Food Industry

基　　金：国家科技基础性工作专项项目(2013FY110400)

摘　　要：为了研究液体培养条件对葡萄状枝瑚菌菌丝生长量的影响,通过单因素实验和正交试验分别考察了碳源、氮源、无机离子组合、生长因子、培养基初始pH、培养温度、摇床转速和光照这8个因素对菌丝生长量的影响,结果表明,最佳培养条件为:在23℃、摇床转速为220 r/min时,培养基初始p H6.5,装瓶量50 mL/250 mL锥形瓶,接种量10%(V/V),全黑暗培养7 d,1 L液体培养最佳培养基为25 g可溶性淀粉,3.5 g酵母粉,4.4 g(1.2 g KH_2PO_4+1.6 g FeSO_4·7H_2O+1.6 g MgSO_4·7H_2O)无机离子,0.01 g VB_1,在此条件下葡萄状枝瑚菌菌丝生长量为(26.8±0.29)g/L。此研究结果为葡萄状枝瑚菌的液体发酵奠定了基础。In order to study the effect of liquid culture conditions on mycelial growth of Ramaria botrytis,eight factors included carbon source,nitrogen source,inorganic ion combination,growth factor,initial p H of culture medium,culture temperature,shaking speed and light were investigated by single factor and orthogonal experiment.The results showed that,the optimum culture conditions were as follows:At 23℃and shaking speed of 220 r/min,initial pH6.5,flask volume 50 mL/250 mL erlenmeyer flask,inoculum density 10%（V/V）,full darkness culture for 7 days,the optimum liquid culture medium for 1L was 25 g soluble starch,3.5 g yeast extract,4.4 g（1.2 g KH2PO4+1.6 g FeSO4·7H2O+1.6 g MgSO4·7H2O）inorganic ion combination and 0.01 g VB1,under the above conditionsthe mycelial growth of R.botrytis was（26.8±0.29）g/L,the result laid the foundation for liquid fermentation of R.botrytis.

关 键 词：葡萄状枝瑚菌 液体培养 营养要素 培养条件 菌丝生长 

分 类 号：TS201.3[轻工技术与工程—食品科学]