桦木酮衍生物缩氨基硫脲金属配合物的合成与表征  

Synthesis and characterization of betulone derivatives of thiosemicarbazones and their metal complexes

作　　者：刘颖[1] 王葛阳 朴凤玉[1] 韩荣弼[1] LIU Ying;WANG Geyang;PIAO Fengyu;HAN Rongbi(Key Laboratory of Natural Resources of Changbai Mountain ~Functional Molecules (Yanbian University),Ministry of Education,Yanji 133002,China)

机构地区：[1]长白山生物资源与功能分子教育部重点实验室(延边大学),吉林延吉133002

出　　处：《延边大学学报（自然科学版）》2018年第3期229-238,共10页Journal of Yanbian University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(21362039)

摘　　要：以桦木醇为起始物,通过对其3号位与28号位进行修饰,合成了两种配体(桦木酮酸缩氨基硫脲和28-O-丁二酸酯桦木酮缩氨基硫脲)及其金属配合物.利用红外、氢谱、质谱、元素分析及热重分析等测试手段对金属配合物进行了表征,并对所合成配体及金属配合物进行了抗菌活性测试与Hela癌细胞毒性测试.通过抑菌活性试验发现:桦木酮酸缩氨基硫脲金属镉、银络合物对S.aureus 4220型和E.coli 1924型细菌有抑制作用,28-O-丁二酸酯桦木酮缩氨基硫脲对大多数细菌都有一定的抑制作用,28-O-丁二酸酯桦木酮对Streptococcus mutans 3289型细菌的抗菌活性与氯霉素相当.通过细胞毒性试验发现:桦木酮酸缩氨基硫脲铜配合物、28-O-丁二酸酯桦木酮缩氨基硫脲及其金属络合物具有良好的抑制Hela细胞的能力,且其抑制率随着化合物浓度的增加而增加;本文所合成的金属络合物抑制Hela细胞的能力优于其配体;在癌细胞毒性测试中的化合物IC50值为10～25μM.Two ligands(betulonic acid thiosemicarbazone and 28-O-succinylbetulone thiosemicarbazone)and their metal complexes have been synthesized using betulin as a starting material via modification of 3-and 28-position of betulin.The complexes were characterized by infrared spectra analysis and proton nuclear magnetic resonance,elemental analysis,matrix-assisted laser desorption ionization mass spectrometry and thermogravimetric analysis.Finally,antibacterial activity test and Hela cancer cell toxicity of the synthesized ligand and metal complexes were performed.The inhibition of S.aureus 4220 and E.coli 1924 was found by antibacterial activity test.28-O-succinylbetulone thiosemicarbazone has a certain inhibitory effect on most bacteria,and 28-O-succinylbetulone has antibacterial activity comparable to chloramphenicol in Streptococcus mutans 3289.The cytotoxicity test showed that betulonic acid thiosemicarbazone copper complex,28-O-succinylbetulone thiosemicarbazone and its metal complex had the ability to inhibit Hela cells,and their inhibition rates increase with the increase of compounds concentration metal complexes synthesized and tested in this paper have better ability to inhibit Hela cells than their ligands.IC50 values of compounds in cytotoxicity tests are 10-25μM.

关 键 词：桦木醇 金属配合物 抑菌活性 细胞毒性 

分 类 号：O629.61[理学—有机化学]