ε-聚L-赖氨酸高产菌株诱变选育  

Screening of High-yieldε-poly-L-lysine Producing Strains Through Mutation Breeding

作　　者：李南臻 王刚[1,2,3] 万玉军 庹有朋[1,2,3] 罗丽娟 岳晓敏 LI Nan-zhen;WANG Gang;WAN Yu-jun;TUO You-peng;LUO Li-juan;YUE Xiao-min(Sichuan Food and Fermentation Industry Research &Design Institute,Wenjiang Chengdu 611130China;Sichuan Engineering Laboratory of Food Preservative Agent by Microbial Fermentation Technology,Wenjiang Chengdu 611130China;Sichuan Food and Fermentation Engineering Technology Center,Wenjiang Chengdu 611130China)

机构地区：[1]四川省食品发酵工业研究设计院,成都温江611130 [2]四川省食品防腐保鲜剂微生物发酵技术工程实验室,成都温江611130 [3]四川食品发酵工程技术中心,成都温江611130

出　　处：《食品与发酵科技》2018年第6期1-4,共4页Food and Fermentation Science & Technology

基　　金：四川省科技支撑项目(2017RZ0030)

摘　　要：微生物发酵生产的广谱抗菌的多肽ε-聚L-赖氨酸(ε-PL),易被生物分解,对人体无害,具有广阔的应用前景,目前已作为保鲜防腐剂在食品加工中使用。本研究立足ε-PL菌株的诱变选育,以白色链霉菌H为出发菌株,利用钴60和ARTP对菌株进行复合诱变,最终选育出ε-PL高产菌株H1-2,突变菌株在10L发酵罐规模,ε-聚L-赖氨酸产量达到24.5g/L,比出发菌株产量提高了88.5%。Epsilon-polylysinehas broad-spectrum antibacterial activity.It is produced by microbial fermentation and easily biodegradable,harmless to human body.It shows broad application prospects.It has been used as food preservative in food processing.This topic mainly study the strain mutation breeding,using Streptomyces albus-H as stating strain,mutant strain with combined Co-60 and ARTP for screening high ε-PL producing strain.Finally,the ε-PL high-producing strain H1-2 was selected,and the mutant strain was in a 10L fermenter scale,the yield of ε-PL reached 24.5 g/L,which was increased 88.5% of starting strain.

关 键 词：白色链霉菌 诱变选育 ε-聚L-赖氨酸 

分 类 号：TQ922.3[轻工技术与工程—发酵工程]