液相色谱-串联质谱测定乳制品中乳酸链球菌素  

Determination of Nisin in Milk Products by HPLC-MS/MS

作　　者：林毅侃[1] 雷涛[1] 陆志芸[1] 庞贝妮 刘天益[1] 段文锋[1] LIN Yikan;LEI Tao;LU Zhiyun;PANG Beini;LIU Tianyi;DUAN Wenfeng(Shanghai Institute of Quality Inspection and Technical Research,Shanghai 200233)

机构地区：[1]上海市质量监督检验技术研究院/国家食品质量监督检验中心,上海200233

出　　处：《食品工业》2018年第12期312-314,共3页The Food Industry

基　　金：上海科学技术委员会科研计划项目"长三角乳及乳制品协同监管服务平台开发及应用示范"(16395810102)

摘　　要：建立液相色谱-串联质谱法用于测定乳酸链球菌素的含量。PLRPS-S为色谱柱。样品经提取净化后,用超高效液相色谱分离后采用电喷雾串联质谱进行定性和定量检测。线性范围为0～1 000μg/L,线性相关系数r大于0.99,方法检出限为0.2 mg/kg,在加标浓度为0.2～5.0 mg/kg条件下的回收率为89.01%～104.52%,精密度为1.99%～8.77%。方法简单、灵敏、准确、精密,满足检测需求。A HPLC-MS/MS method for the determination of Nisinin milk products has been established. The samples were analyzed by the separation on a PLRPS-S chromatographic column, and determined by HPLC-MS/MS. The calibration curves of Nisin showed a good linearity in range of 0-1 000 μg/L with correlation of more than 0.99, and the detection limits were 0.2 mg/kg. The average recovery was 89.01%-104.52%. The relative standard deviation was 1.99%-8.77%. This method was simple, sensitive, accurate and precise, and it could be used for determination of Nisin in milk products.

关 键 词：液相色谱-串联质谱 乳制品 乳酸链球菌素 测定 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]