雨生红球藻中虾青素含量快速测定方法  

A rapid determination method for astaxanthin content in Haematococcus pluvialis

作　　者：王书妍[1] 曹旭鹏[2] 孟迎迎 刘娇[2] 薛松[2] WANG Shuyan;CAO Xupeng;MENG Yingying;LIU Jiao;XUE Song(Chemistry and Chemical Engineering College,Inner Mongolia University for Nationlities,Tongliao 028000,Inner Mongolia,China;Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 115023,Liaoning,China;College of Chemistry,Chemical Engineering and Materials Science,Shandong Normal University,Jinan 250014,China)

机构地区：[1]内蒙古民族大学化学化工学院,内蒙古通辽028000 [2]中国科学院大连化学物理研究所,辽宁大连116023 [3]山东师范大学化学化工与材料科学学院,济南250014

出　　处：《中国油脂》2018年第12期144-148,共5页China Oils and Fats

基　　金：国家海洋局海洋公益性行业科研专项经费项目(201505030-1);内蒙古教育厅项目(NJZY168)

摘　　要：雨生红球藻中虾青素以虾青素单酯、二酯以及少量游离虾青素的形式存在,为了准确测定虾青素含量,通常需要将提取的虾青素酯水解转化为游离虾青素,再利用HPLC进行定量,操作耗时,不利于生产过程的快速监测。基于系统研究分光光度法直接测定细胞提取物中的混合虾青素含量和提取-酶解-HPLC法测定的关系,发现分光光度法估算的虾青素含量与HPLC法测定的准确含量之间具有良好的线性关系(r2=0. 997)。基于此建立了雨生红球藻虾青素快速测定方法,并对提取条件进行了优化。雨生红球藻粉(约5 mg)利用1 m L二甲基亚砜和6 m L丙酮进行1次提取,准确定容后,测定474 nm处的吸光度,根据吸光度与HPLC法虾青素含量间线性关系计算雨生红球藻中虾青素的含量。该方法操作简单,仅需10～20 min,测定准确,适于生产和流通环节的所需要的快速测定领域。Astaxanthin existed in the forms of astaxanthin esters and a small amount of free astaxanthin in Haematococcus pluvialis. The astaxanthin esters should be hydrolyzed to free astaxanthin before quantitation by HPLC,which was time consuming process and not conducive to the rapid monitoring of the production process. The relationship of determination of astaxanthin content direct by spectrophotometry and extraction-enzymatic hydrolysis-HPLC method was studied systematically. It was found that there was a good linear relationship between astaxanthin content estimated by spectrophotometry and determined by HPLC( r2= 0. 997). A rapid determination method for astaxanthin content in Haematococcus pluvialis was established,and the extraction conditions were optimized. The powder of Haematococcus pluvialis( about5 mg) was extracted by 1 m L dimethylsulfoxide( DMSO) and 6 m L acetone. After constant volume,it was determined at 474 nm by spectrophotometry,and the astaxanthin content was calculated according to the linear relationship between absorption and astaxanthin content determined by HPLC. The method had advantages of simple operation and accurate measurement,which only needed 10-20 min and only used spectrophotometry. The method was suitable for rapid determination in the production and circulation links.

关 键 词：雨生红球藻 虾青素 快速测定 分光光度法 

分 类 号：Q819[生物学—生物工程] O657[理学—分析化学]