MADS-box 转录因子编码基因 Vvrin1在草菇不同发育时期的表达分析  

Expression analyses of Vvrin1 gene encoding MADS-box transcription factors in different development stages of Volvariella volvacea

作　　者：陈炳智[1,2] 李凌 钟千贵 潘欣圆 谢宝贵[2] 江玉姬[1,2] CHEN Bing-Zhi;LI Ling;ZHONG Qian-Gui;PAN Xin-Yuan;XIE Bao-Gui;JIANG Yu-Ji(College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]福建农林大学菌物研究中心,福建福州350002

出　　处：《菌物学报》2018年第12期1628-1634,共7页Mycosystema

基　　金：国家自然科学基金(31801920);国家现代农业产业技术体系建设专项资金(CARS24);国家重点基础研究发展计划(2014CB138302);福建农林大学科技创新基金(CXZX2017410).

摘　　要：草菇是我国土特产出口的主要种类之一,而采后易开伞问题限制了草菇的贮藏及运输。MADS-box转录因子对植物的成熟衰老和真菌的子实体发育起到重要的调控作用。目前尚未见草菇MADS-box转录因子的相关报道。本研究通过生物信息方法及分子生物学的手段对草菇的基因组、转录组数据进行分析,获得了草菇MADS-box转录因子基因Vvrin1。该基因全长1 392bp,含2个内含子,编码419个氨基酸残基。该转录因子含有一个MADS-box结构域,序列与双孢蘑菇Agaricusbisporus、斑玉蕈Hypsizygusmarmoreus和灰盖鬼伞Coprinopsiscinerea的MADS-box转录因子相似性分别为71%、67%和61%。通过表达谱数据及荧光定量PCR分析表明Vvrin1基因在草菇子实体伸长期菌柄的表达量出现高峰,具有极显著性差异(P<0.01),推测该转录因子参与调控草菇菌柄的伸长,菌盖的开伞。这些结果为草菇成熟衰老(特别是开伞)的调控研究提供数据支持。Volvariella volvacea is one of the main types of export of local products in China.The pileus of V.volvacea fruiting bodies were easily expanded after harvest which limited the storage life and transportation distance.MADS-box transcription factor plays an important role in the regulation of plant maturation and senescence as well as the development of fungal fruiting body.At present,no relevant studies on the MADS-box transcription factors of V.volvacea have been reported.In this study,one gene Vvrin1 encoding MADS-box transcription factor of V.volvacea was identified by means of bioinformatics and molecular biology analysis on genome and transcriptome data.Vvrin1 gene is 1 392bp,containing two introns and encoding 419 amino acids.The protein contains an MADS-box domain,with sequences similar to MADS-box proteins of Agaricus bisporus,Hypsizygus marmoreus and Coprinopsis cinerea at resemblance of 71%,67% and 61%,respectively.Digital gene expression(DGE)data and qRT-PCR data demonstrated that the expression of Vvrin1 gene in the stipe elongation stage was highest in fruiting period,showing significant difference(P<0.01).It was speculated that this transcription factor was involved in the regulation of the elongation of the stipe and the pileus expansion in V.volvacea.This study provides data supporting the regulation research of mushroom maturation and senescence,especially pileus expansion.

关 键 词：中国蘑菇 MADS-box结构域 开伞 成熟衰老 

分 类 号：S646.13[农业科学—蔬菜学]