HBVpreC/C区基因突变与原发性肝癌预后的关系  

Relationship between the mutations in precore/core region of hepatitis B virus gene and the postoperative survival in hepatocellular carcinoma

作　　者：吴忱思[1] 吴建华[2] 赵乐 王英南[1] 张风宾[1] 张卫国[3] 张瑞星[1] Wu Chensi;Wu Jianhua;Zhao Yue;Wang Yingnan;Zhang Fengbin;Zhang Weiguo;Zhang Ruixing(Department of Gastroenterology,The Fourth Hospital of Hebei Medical University,Shijiazhang 050011,China;The Center of Laboratory Animal,The Fourth Hospital of Hebei Medical University,Shifiazhang 050011,China;Department of Clinical Focus, Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院消化内科,石家庄050011 [2]河北医科大学第四医院实验动物中心,石家庄050011 [3]河北医科大学《临床荟萃》编辑部,石家庄050011

出　　处：《中华实验和临床病毒学杂志》2018年第6期571-575,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：河北省医学科学研究重点课题计划(20180494);河北省科技厅重点研发计划(152777148).

摘　　要：目的研究乙型肝炎病毒(hepatitis B virus,HBV)preC/C区基因突变与原发性肝癌患者预后的关系。方法收集81例乙型肝炎病毒相关性肝细胞肝癌(hepatitis B virus associated hepatocellular carcinoma,HBV-HCC)患者的癌组织并提取基因组DNA,对HBVpreC/C区基因进行扩增和测序,并根据NCBI数据库鉴定出其突变位点,运用Kaplan-Meier和Cox回归等方法分析HBV-HCC患者的临床资料、突变位点与其术后生存期之间的关系。结果门脉瘤栓、肿瘤分期和肿瘤大小是与HBV-HCC患者术后生存相关的独立危险因素。1915、2134、2176、2221和2260突变位点被确定为预测HBV-HCC患者生存相关的独立危险因子,1979和2245突变位点与HBV-HCC患者生存具有临界统计学差异。结论门脉瘤栓、肿瘤分期和肿瘤大小以及HBVpreC/C区基因的以上7个突变位点被确定为与肝癌患者术后预后相关的独立危险因素。Objective To investigate the relationship between the mutations in precore/core (preC/C) region of hepatitis B virus (HBV) gene and the postoperative survival in patients with hepatocellular carcinoma. Methods A total of 81 cases in HBV associated hepatocellular carcinoma (HBV-HCC) patients with cancer tissue genomic DNA were extracted. The preC/C region of HBV was amplified and sequenced, and survival-associated HBV mutations were identified according to the NCBI database. The relationships between the mutations in the preC/C region and HCC survival was analyzed with the Kaplan-Meier method and the Cox proportional hazards model. Eleven mutational sites were identified as statistically significant independent predictors of HBV-HCC postoperative survival. Results The portal vein thrombosis, tumor TNM classification and size were identified as statistically significant independent predictors of survival in HBV-HCC patients. In the research, we found that seven mutational sites in preC/C region of HBV were associated with independent risk factors for postoperative survival in patients of HBV-HCC. The following five mutational sites were identified as statistically significant independent predictors of HBV-HCC survival: 1915, 2134, 2176, 2221, 2260. The mutational site of 1979 and 2245 were identified for the association with survival at a borderline significance level. Conclusions The portal vein thrombosis, tumor TNM classification, size and seven mutational sites in the PreC/C region were identified as independent predictors of postoperative survival in HCC patients.

关 键 词：HBV-HCC HBV preC/C区 HBV-DNA 突变位点 预后 

分 类 号：R735.7[医药卫生—肿瘤]