枝瑚菌多糖的结构及其免疫调节活性  

Structure and Immunomodulatory Activity of Polysaccharide from Ramaria flaccida(Fr.) Quél

作　　者：董明明 丁祥[1,2] 宋波 蒋琳[1] 刘影 侯怡铃[1] DONG Ming-ming;DING Xiang;SONG Bo;JIANG Lin;LIU Ying;HOU Yi-ling(Key Laboratory of Southwest China Wildlife Resources Conservation,College of Life Sciences,China West Normal University,Nanchong 637009,China;College of Environmental Science and Engineering,China West Normal University,Nanchong 637009,China)

机构地区：[1]西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室,四川南充637009 [2]西华师范大学环境科学与工程学院,四川南充637009

出　　处：《食品工业科技》2019年第1期66-71,76,共7页Science and Technology of Food Industry

基　　金：四川省科技厅应用基础重点项目(2018JY0087);四川省科技厅重点研发项目(2018NZ0055)

摘　　要：本研究以枝瑚菌中分离纯化的枝瑚菌多糖(RF-S)为研究对象,运用红外光谱技术研究了其结构;运用细胞学技术,从体外水平,以巨噬细胞和淋巴细胞为靶细胞,研究了RF-S的免疫调节活性。结果表明:RF-S多糖结构明显,无其他结构,为多糖纯品,且其多糖中的单糖是以吡喃糖苷的形式存在。随着RF-S质量浓度的增加,极显著促进巨噬细胞、T淋巴细胞和B淋巴细胞的增殖(p <0.01),并呈现一定的剂量依赖关系。同时,当RF-S质量浓度为5μg/m L时,能极显著地促进巨噬细胞吞噬中性红和荧光微球(p <0.01),并能极显著地刺激巨噬细胞分泌IL-2、IL-10、TNFα和释放NO(p <0.01);同时对刺激巨噬细胞分泌IL-6有显著效果(p <0.05)。综上所述,RF-S具有良好的免疫调节活性。In this study,polysaccharides(RF-S) was isolated from the Ramaria flaccida(Fr.) Quél,which was used as the research object of study.Its structure was studied by infra-red spectrum,and the immunomodulatory activity of RF-S was studied in vitro by using cytological techniques and using macrophages and lymphocytes as target cells.The results showed that the polysaccharide structure of RF-S was typical without any other structure,and it was a kind of pure polysaccharide.And the monosaccharide in its polysaccharides existed in the form of pyranose.In addition,with the increase of RF-S concentration,the proliferation of macrophages,T lymphocytes and B lymphocytes could be extremely significantly promoted(p < 0.01),and showed a dose-dependent relationship.Meanwhile,when the mass concentration of RF-S was 5 μg/m L,it could extremely significantly promote phagocytosis of neutral red and fluorescent microspheres in macrophage,and could extremely significantly stimulate macrophages to secrete IL-2,IL-10,TNF alpha and release NO(p < 0.01).It also had a significant effect on stimulating the secretion of IL-6 in macrophages(p < 0.05).In conclusion,polysaccharides from the Ramaria flaccida(Fr.)Quél had good immunomodulatory activity.

关 键 词：枝瑚菌 多糖 巨噬细胞 淋巴细胞 免疫调节活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]