块菌多糖复合酶法提取工艺优化  

Optimization of the extraction of truffle polysaccharide by multi-enzyme hydrolysis

作　　者：清源 陈甜甜 QING Yuan;CHEN Tian-tian(Department of Agricultural Science of Xi Chang University,Xichang,Sichuan 615013,China;School of Food and Bioengineering of Xihua University,Chengdu,Sichuan 610000,China)

机构地区：[1]西昌学院农业科学学院,四川西昌615013 [2]西华大学食品与生物工程学院,四川成都610000

出　　处：《食品与机械》2018年第10期174-178,共5页Food and Machinery

基　　金：西昌市科技项目(编号:15YYJS001);西昌学院博士研究生启动项目(编号:15BZ03)

摘　　要：以印度块菌为原料,运用复合酶法提取块菌多糖。采用单因素和均匀试验设计方法,考察酶配比、酶解时间、酶解温度、酶用量、料液比、pH值6个因素对块菌多糖得率的影响。按照U_(10)(10~8)进行试验,考察各因素及其交互作用对块菌多糖得率的影响,预测和验证最佳工艺参数。结果表明:块菌多糖提取的最佳工艺条件为中性蛋白酶与纤维素酶1:7(g/g)、酶解时间48min、酶解温度41℃、酶用量3.6%、料液比1:29 (g/mL)、酶解pH值4.8,多糖得率可达14.50%,含量可达75.96%。经验证,该工艺稳定可行,适用于块菌多糖的提取。The polysaccharide was extracted by compound enzyme method from the Indian truffle.Through single factor and uniform design experiments,the effect on the yield of truffle polysaccharide were investigated,including the enzyme ratio,zymolytic time and temperature,enzyme dosage,material/liquid ratio and pH value.According to U10(108)experiment,the effects of six single factors as well as their interactions on the yield of polysaccharide were studied.The optimal process parameters were predicted and verified.The results showed that the best extraction conditions of truffle polysaccharide was as follows:dispase and cellulase ratio of 1:7(g/g),zymolytic time 48 min,zymolytic temperature 41 ℃,enzyme dosage3.6%,material/liquid ratio 1:29(g/mL)and pH 4.8.The yield of polysaccharide was up to 14.50%,and the content of polysaccharide could reach 75.96%.The confirmatory tests proved that the optimal conditions were reliable and suitable for extracting truffle polysaccharides.

关 键 词：块菌 多糖 复合酶法 

分 类 号：TQ281[化学工程—有机化工]