驱动蛋白家族成员15在肝癌中的表达及意义  

Expression of kinesin family member 15 in liver cancer and its significance

作　　者：何政[1] 吴慧[1] 郑军[1] HE Zheng;WU Hui;ZHENG Jun(Department of Hepatopancreatobiliary Surgery,Yichang Central People's Hospital,the First College of Clinical Medical Science,Three Gorges University,Yichang,Hubei 443002,China)

机构地区：[1]三峡大学第一临床医学院宜昌市中心人民医院肝胆胰外科,湖北宜昌443002

出　　处：《中国普通外科杂志》2019年第1期58-63,共6页China Journal of General Surgery

基　　金：湖北省卫计委科研基金资助项目(WJ2015MB182)

摘　　要：目的:探究驱动蛋白家族成员15(KIF15)在肝癌中的表达以及与肝癌生物学行为的关系。方法:利用TCGA数据分析不同临床情况肝癌患者KIF15表达及其对肝癌患者生存率的影响。观察肝癌SMMC-7721细胞的KIF15表达下调后,增殖能力、细胞周期及侵袭能力的变化。结果:数据库分析结果显示,肝癌患者的TNM或T分期越高,KIF15相对表达量越高(均P<0.05);KIF15高表达肝癌患者生存率明显低于KIF15低表达肝癌患者(P<0.05)。SMMC-7721细胞转染siKIF15后,细胞增殖活性明显降低、细胞G_0/G_1期阻滞明显增加、细胞侵袭能力明显减弱。结论:KIF15的表达于肝癌的进展密切相关,其作用机制可能与促进肝癌细胞增殖和侵袭有关。Objective: To investigate the expression of kinesin family member 15(KIF15) in liver cancer and its relation with the biological behaviors of liver cancer cells. Methods: Using TCGA database, the KIF15 expressions in liver cancer patients with different clinical conditions and its association with the survival rates of liver cancer patients were analyzed. In liver cancer SMMC-7721 cells after down-regulating KIF15 expression, the changes in proliferative ability, cell cycle characteristics and invasion capacity were observed. Results: The results of database analysis showed that the more advanced TNM or T stages corresponded to higher KIF15 expression(both P<0.05);The survival rate in liver cancer patients with higher KIF15 expression was significantly lower than that in liver cancer patients with low KIF15 expression(P<0.05). In SMMC-7721 cells after transfection with siKIF15, the proliferative ability was significantly reduced, G0/G1 phase arrest was significantly increased, and the invasion capability was significantly weakened(all P<0.05). Conclusion: KIF15 expression is closely related to the progression of liver cancer, and the mechanism may probably be associated with its promoting the proliferation and invasion of the liver cancer cells.

关 键 词：癌 肝细胞 驱动蛋白 计算生物学 肿瘤侵润 

分 类 号：R735.7[医药卫生—肿瘤]