马尾藻岩藻聚糖分离纯化及对HepG2细胞胆固醇含量的影响  

Separation and purification of fucoidan from Sargassum and its effect on cholesterol in HepG2 Cells

作　　者：刘海韵 谌素华[1,2] 王维民 黄娟娟[1] 廖森泰 LIU Hai-yun;CHEN Su-hua;WANG Wei-min;HUANG Juan-juan;LIAO Sen-tai(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang,Guangdong 524088,China;Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Zhanjiang ,Guangdong 524088,China;Sericulture & Farm Produce Processing Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou ,Guangdong 510610,China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东省水产品加工与安全重点实验室,广东湛江524088 [3]广东省农业科学院蚕业与农产品加工研究所,广东广州510610

出　　处：《食品与机械》2018年第11期135-139,共5页Food and Machinery

基　　金：广东省自然科学基金项目(编号:2015A030313618);2015年广东省联合培养研究生示范基地项目(编号:粤教研函[2016]1号)

摘　　要：采用DEAE C-52纤维素阴离子交换层析法对亨氏马尾藻岩藻聚糖进行分级纯化,将得率最高的组分命名为HF1,对其进行自由基氧化降解得到HDF1和HDF2,取回收率较高的HDF2采用Superdex 75凝胶柱层析法分离纯化得到HDF21和HDF22 2种组分。体外培养HepG2细胞,将不同浓度的各组分作用于HepG2细胞,测定细胞内胆固醇含量。结果表明:经过降解,HDF1的多糖含量、硫酸基含量和岩藻糖含量均高于HF1,但糖醛酸含量低于HF1。降解作用对HDF21的化学成分影响最大。HF1、HDF1、HDF21、HDF22 4种组分作用于HepG2细胞后均能降低细胞内胆固醇含量,并且在高浓度(500μg/mL)时效果最好;高浓度组作用于细胞72h后细胞内胆固醇含量均极显著降低(P<0.01),并且以HDF21降胆固醇效果最佳。DEAE C-52 cellulose anion exchange chromatography was used to fractionate and purify the fucoidan fromSargassum henslowianum.The highest yielding fraction was named HF1.And free radical oxidative degradation was used to obtain HDF1 and HDF2.The higher HDF2 was separated and purified using Superdex 75 gel column chromatography to obtain HDF21 and HDF22 components.HepG2 cells were cultivated in vitro and it were affected with different concentrations of each component to determine the intracellular cholesterol content.The results showed that after degradation,the polysaccharide content,sulfate content and fucose content of HDF1 were higher than HF1,but the uronic acid content was lower than HF1.The degradation had the greatest influence on the chemical composition of HDF21.The four components of HF1,HDF1,HDF21 and HDF22 could reduce the intracellular cholesterol content after HepG2 cells were treated,and the best effect was at high concentration(500μg/mL).After 72 hours of treatment in high concentration group,the intracellular cholesterol content was decreased most significantly(P<0.01),and HDF21 had the best cholesterol-lowering effect.

关 键 词：马尾藻 岩藻聚糖 分离 纯化 降解 HEPG2细胞 胆固醇 

分 类 号：TS201.2[轻工技术与工程—食品科学]