海带多糖对喉癌细胞增殖和凋亡影响的实验探索  

Effect of laminaria japonica polysaccharides on laryngeal carcinoma cell proliferation and apoptosis in vitro

作　　者：邹平[1] 胡国文[1] 刘跃辉[1] ZOU Ping;HU Guowen;LIU Yuehui(Department of Otolaryngology,People's Hospital of Gaoming District,Foshan,Guangdong,528500,China)

机构地区：[1]佛山市高明区人民医院耳鼻咽喉科,广东佛山528500

出　　处：《中国耳鼻咽喉头颈外科》2019年第1期13-16,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的通过海带多糖干预人喉癌Tu212细胞系,探索其对Tu212细胞增殖和凋亡的影响及其抗肿瘤活性机制。方法不同浓度的海带多糖(80、160、320μg/ml)及顺铂(1.8 mg/L)分别作用于喉癌Tu212细胞,采用MTT比色法检测24、48、72小时后的生长抑制率;流式细胞仪检测48小时后Tu212细胞各期的生长情况;荧光显微镜观察48小时后Tu212细胞的凋亡情况;Western Blot检测CyclinBl,Bcl-2蛋白的表达情况。结果随着海带多糖浓度的增加,其对喉癌Tu212细胞的抑制率也升高,相同浓度的海带多糖对喉癌Tu212细胞的抑制率随着时间的延长而升高;海带多糖干预Tu212细胞48小时后,其增殖被阻滞在G2/M期;干预48小时后,荧光显微镜观察可见典型细胞凋亡;干预Tu212细胞48小时后,CyclinBl,Bcl-2蛋白表达下降,差异均有统计学意义。结论海带多糖对喉癌Tu212细胞的生长有抑制作用,其机制为通过抑制增殖及促进凋亡来实现。OBJECTIVE To investigate the effect and its mechanism of laminaria japonica polysaccharides(LJP) on human laryngeal carcinoma cell Tu212 growth. METHODS The laryngeal squamous cell Tu212 in logarithmic growth phase were treated by laminaria japonica polysaccharides(80,160, 320 μg/ml) and cisplatin(1.8 mg/L). The MTT assay was used to evaluate cell growth inhibition rate after 24 h, 48 h and 72 h. The flow cytometry with PI staining were used to estimate cell cycle distribution after 48 h. The fluorescent microscopy was used to estimate cell apoptosis after 48 h. The western blot was used to evaluate the expressions of Cyclin B1 and Bcl-2. RESULTS The cell proliferation was inhibited by cellular LJP in a dose and time dependent manner. Tu212 cells proliferation were stopped at the G2/M phase treated with LJP after 48 h. Cell apoptosis was observed clearly by fluorescent microscope after 48 h. The expressions of Cyclin B1 and Bcl-2 were suppressed significantly treated with LJP after 48 h. All of the differences were significant(P<0.05). CONCLUSION LJP has inhibitive effects on the Tu212 cells, this mechanism was probably through inhibition of the proliferation and promotion of the apoptosis.

关 键 词：喉肿瘤 细胞增殖 细胞凋亡 海带多糖 Tu212细胞 

分 类 号：R739.65[医药卫生—肿瘤]