2017年辽宁省人感染汉坦病毒G2片段基因序列特征分析  

Analysis on gene sequence characteristic of hantavirus G2 segment in patients with hemorrhagic fever with renal syndrome in Liaoning,2017

作　　者：车雷[1] 张国斌[1] 高丹[1] 赵梁[1] 王晓丽[1] Che Lei;Zhang Guobin;Gao Dan;Zhao Liang;Wang Xiaoli(Shenyang Municipal Centre Disease Control and Prevention,Shenyang 110031,Liaoning,China)

机构地区：[1]沈阳市疾病预防控制中心,辽宁沈阳110031

出　　处：《疾病监测》2018年第12期995-999,共5页Disease Surveillance

基　　金：沈阳市科技计划项目(No.F16–206–9–36)~~

摘　　要：目的了解2017年辽宁省肾综合征出血热(HFRS)患者感染汉坦病毒的(HV)的基因型别及变异情况。方法采集HFRS患者急性期血清,采用酶联免疫吸附试验筛选IgM抗体阳性标本,从阳性标本全血中提取HV的RNA,通过反转录聚合酶链式反应(RT-PCR)扩增G2基因片段并进行基因分型,对扩增产物进行核苷酸序列测定,利用DNAStar软件包分析。结果 10份标本经RT-PCR提取扩增出的目的片段,4份为汉滩型(HTN型),6份为汉城型(SEO型)。扩增产物测序结果与已测得国内毒株及部分国际标准株比较,4份HTN型HV之间核苷酸同源性达到95.6%～98.9%。与HTN76–118株同源性最高,为89.3%～90.1%,氨基酸同源性为98.4%～99.2%。6份SEO型HV间核苷酸同源性高达98.3%～99.8%。与80–39株同源性最高,为96.3%～96.6%,推导氨基酸同源性为99.3%～99.6%。结论辽宁省是以SEO型HV为主的SEO型和HTN型混合型疫区,病毒G2基因片段间高度同源,无明显变异,遗传物质相对稳定。Objective To understand the genotype and genovariation of hantavirus isolated from the patients with hemorrhagic fever with renal syndrome(HFRS) in Liaoning province in 2017.Methods Blood samples of HFRS patients in acute phase were collected,the IgM positive serum samples were screened with ELISA and hantavirus RNA was extracted by using the whole blood samples of positive cases.The G2 segment were amplified by RT-PCR and genotyped.The representative hantavirus strains were sequenced and gene analysis was carried out with DNAStar package.Results Ten hantavirus strains isolated were amplified by using RT-PCR,the results indicated that 4 strains belonged to HTN type and the other 6 strains belonged to SEO type.The sequencing results of the products were compared with domestic strains and some international standard strains.The nucleotide homology of 4 HTN type strains was 95.6%–98.9%,and they shared highest nucleotide homology with HTN76–118 strain(89.3%–90.1%).The nucleotide homology of 6 SEO type strains was98.3%–99.8%,and they shared highest nucleotide homology with 80–39 strain(96.3%–96.6%).Their amino homology might be 99.3%–99.6%.Conclusions Both SEO virus and HTN virus circulated in Liaoning province,but HTN was predominant.The G2 segment of hantavirus is highly homologous and has less variation.The genetic materials were relatively stable.

关 键 词：汉坦病毒 G2片段 系统进化树 基因变异 

分 类 号：R373.32[医药卫生—病原生物学]