菊粉复配灵芝多糖对HepG2细胞胰岛素抵抗的调节作用  

Regulating effect of inulin combined with ganoderma lucidum polysaccharide on insulin-resistance of HepG2 cell

作　　者：刘亚萍 李雨蒙[1] 王梦 张文菱子 张泽生[1,2,3] LIU Ya-ping;LI Yu-meng;WANG Meng;ZHANG Wen-ling-zi;ZHANG Ze-sheng(Institute of Food Engineering and Biotechnology,Tianjin University of Science and Technology, Tianjin 300457;State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science &Technology,Tianjin 300457;Tianjin Food Safety &Low Carbon Manufacturing Collaborative Innovation Center,Tianjin 300457)

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457 [2]天津科技大学省部共建食品营养与安全国家重点实验室,天津300457 [3]天津食品安全低碳制造协同创新中心,天津300457

出　　处：《中国食品添加剂》2018年第12期116-121,共6页China Food Additives

摘　　要：目的:研究菊粉复配灵芝多糖对HepG2细胞胰岛素抵抗的调节作用。方法:采用高糖高脂培养HepG2细胞,建立胰岛素抵抗模型(IR-HepG2细胞模型)后,用菊粉、灵芝多糖及其复配物继续培养细胞24h,测定细胞葡萄糖消耗量、糖原合成量、细胞内超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)活力。结果:与模型组相比,菊粉和灵芝多糖均可显著提高IR-HepG2细胞的葡萄糖消耗量、糖原合成量、SOD和GSH-Px活力,并呈现剂量依赖性。将菊粉和不同浓度的灵芝多糖复配之后,与单一的菊粉组相比,复配高剂量组的葡萄糖消耗量、糖原含量、SOD和GSH-Px活力均极显著升高(P <0.01)。结论:菊粉复配灵芝多糖可显著促进IR-HepG2细胞葡萄糖的利用,增加糖原合成,提高SOD和GSH-Px酶活力,改善糖代谢紊乱。Objective :To investigate the effect of inulin combined with ganoderma lucidum polysaccharides on insulin-resistance of HepG2 cells. Methods :The insulin-resistant models of HepG2 cells(IR-HepG2)were induced by high concentration of glucose and palmitic acid for 36 hours. Then,the inulin and ganoderma lucidum polysaccharides were added into the cultured medium. After 24 h hours,the glucose consumption,the glycogen synthesis,superoxide dismutase(SOD)and Glutathione peroxidase(GSH-Px)activities of each group cells were measured. Results :Compared with the model group,the glucose consumption,the glycogen synthesis,SOD and GSH-Px activities of inulin groups and ganoderma lucidum polysaccharides groups increased significantly(P< 0.05). Compared with the single inulin group,the glucose consumption,the glycogen synthesis,SOD and GSH-Px activities of combined groups increased significantly(P < 0.01). Conclusion :The inulin combined with ganoderma lucidum polysaccharides can improve the glucose consumptions,increase glycogen synthesis,and enhance SOD and GSH-Px activities in insulin-resistance model of HepG2 cells.

关 键 词：菊粉 灵芝多糖 复配 HEPG2细胞 胰岛素抵抗 

分 类 号：S567.31[农业科学—中草药栽培] TQ467.32[农业科学—作物学]