Novel strategy of constructing fluorescent probe for MAO-B via cascade reaction and its application in imaging MAO-B in human astrocyte  

作　　者：Huihuan Qin Lingling Li Kun Li Xiaoqi Yu 

机构地区：[1]Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University

出　　处：《Chinese Chemical Letters》2019年第1期71-74,共4页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (Nos. 21572147 and 21472131)

摘　　要：To detect monoamine oxidase B(MAO-B), the level of which is one of the most important indicators of neurodegenerative diseases such as Parkinson's disease, a new type of cascade reaction based on the formation of coumarin was applied in this research. After the reaction with MAO-B, the protecting group of hydroxyl group in probes(DEAN-MA and DEAB-MA) was removed, and the fluorescence intensity significantly increased(l_(em) 456 nm) as the structure of coumarin was formed subsequently. The probes showed excellent sensitivity and selectivity to MAO-B. The detection limit of DEAN-MA and DEAB-MA were 0.6ng/mL and 0.2 ng/mL, respectively. We succeeded in detecting MAO-B in vitro and imaging it in human astrocyte(U87).To detect monoamine oxidase B(MAO-B), the level of which is one of the most important indicators of neurodegenerative diseases such as Parkinson's disease, a new type of cascade reaction based on the formation of coumarin was applied in this research. After the reaction with MAO-B, the protecting group of hydroxyl group in probes(DEAN-MA and DEAB-MA) was removed, and the fluorescence intensity significantly increased(l_(em) 456 nm) as the structure of coumarin was formed subsequently. The probes showed excellent sensitivity and selectivity to MAO-B. The detection limit of DEAN-MA and DEAB-MA were 0.6ng/mL and 0.2 ng/mL, respectively. We succeeded in detecting MAO-B in vitro and imaging it in human astrocyte(U87).

关 键 词：MONOAMINE oxidase-B Fluorescent probe Covalent-assembly COUMARIN Cell IMAGING 

分 类 号：O6[理学—化学]