红曲对ApoE^(-/-)小鼠肠道屏障功能影响  

Effect of Chinese red yeast rice on intestinal barrier function in ApoE^(-/-) rats

作　　者：程慧敏 刘曼[1] 杜威[1] 刘颖[2] 梁惠[1] CHENG Hui-min;LIU Man;DU Wei(Department of Nutrition and Food Hygiene,College of Public Health, Qingdao University,Qingdao,Shandong Province 266021,China)

机构地区：[1]青岛大学公共卫生学院营养与食品卫生教研室,山东青岛266021 [2]青岛大学基础医学院

出　　处：《中国公共卫生》2019年第2期171-175,共5页Chinese Journal of Public Health

基　　金：国家自然科学基金(81573131);汤臣倍健营养科学研究基金(2016-102)

摘　　要：目的研究红曲对ApoE^(-/-)小鼠肠道屏障功能的影响。方法 6只雄性8周龄C57BL/6J小鼠作为正常对照组,18只8周龄雄性ApoE^(-/-)小鼠随机分为:动脉粥样硬化模型组;他汀对照组;红曲组。实验周期为12周。主动脉大体油红O染色,定量斑块面积;苏木精-伊红染色法(HE)观察结肠病理变化;透射电镜观察结肠超微结构变化;免疫印迹法(WB)检测结肠组织中紧密连接蛋白ZO-1、claudin-1的表达水平。结果正常对照组无斑块形成,动脉粥样硬化模型组斑块面积为(22.46±8.33)%。与正常对照组相比,模型组结肠绒毛高度和微绒毛长度[分别为(66.27±6.38)、(0.67±0.19)μm]降低(P <0.05);与模型组相比,红曲组结肠绒毛高度和微绒毛长度[(92.57±7.37)、(0.87±0.07)μm]均升高(P<0.05)。与对照组相比,模型组结肠组织中claudin-1和ZO-1蛋白表达水平[分别为(494.00±47.15)、(466.67±43.41)]降低(P <0.05);与模型组相比红曲组claudin-1和ZO-1蛋白表达水平[(709.33±60.00)、(785.67±101.55)]均升高(P<0.05)。红曲组所测各指标和他汀组相比均无显著性差异(P>0.05)。结论红曲可以改善ApoE^(-/-)小鼠肠道屏障功能,其机制可能与修复结肠绒毛和通过上调结肠ZO-1和claudin-1紧密连接蛋白有关。Objective To study the effect of red yeast rice(monacolin K) on intestinal barrier function in ApoE-/-rats.Methods Eighteen 8-week-old male ApoE-/- mice were fed with feeds containing 15% lard and 0.25% cholesterol to establish atherosclerosis model and then randomly divided into a model(with daily gavage of saline), a statin control(10 mg/kg.d statin), and a red yeast rice group(0.34 g/kg.d monacolin K) and 6 male 8-week-old C57 BL/6 J rats were used as normal control for a 12-week experiment. The aorta specimens were stained with oil red O to quantify the area of plaque;pathological changes of the small intestine were observed with hematoxylin-eosin(HE) staining;the ultrastructure of the small intestine was observed with transmission electron microscopy;and Western blot(WB) was used to detect the expression of tight junction protein zonula occludens-1(ZO-1) and claudin-1 in colon tissues. Results No aorta plaque formation was observed in the normal control group. The average aorta plaque area for the atherosclerosis model group was22.46 土 8.33%. Compared with the normal control group, the mean height of colonic villi(66.27 ± 6.38 μm) and the mean length of colonic microvilli(0.67 ±0.19 μm) of the model group were significantly decreased compared with those of the normal control group(both P < 0.05) and the mean height of colonic villi(92.57 ± 7.37 μm) and the length of colonic microvilli(0.87 ± 0.07 μm) of the monacolin K group increased significantly(both P < 0.05) in contrast to those of the model group. The protein expression of claudin-1(494 ± 47.15) and ZO-1(466.67 ± 43.41) of the model group were significantly lower than those of the normal control group(both P< 0.05). Compared with the model group, the protein expression level of claudin-1(709.33 ± 60) and ZO-1(785.67 ± 101.55) in the colonic tissues of the monacolin K group increased significantly in comparison with those of the model group(both P < 0.05). There were no significant differences in all the indicators between monacolin K grou

关 键 词：红曲 动脉粥样硬化 肠道屏障 紧密连接蛋白类 

分 类 号：R151.2[医药卫生—营养与食品卫生学]