HS-GC-MS/MS法检测海产品中的7种苯系物  

Determination of 7 benzene series in seafood by headspace GC-MS/MS

作　　者：周秀锦 杨会成 邵宏宏 张静 相兴伟 韩超 郑斌 ZHOU Xiu-jin;YANG Hui-cheng;SHAO Hong-hon1;ZHANG Jing;XIANG Xing-wei;HAN Chao;ZHENG Bin(Zhoushan Entry-exit Inspection and Quarantine Bureau,Zhoushan 316000;Zhejiang Marine Development Research Institute,Zhoushan 316000;Wenzhou Entry-exit Inspection and Quarantine Bureau,Wenzhou 325000)

机构地区：[1]舟山出入境检验检疫局,舟山316000 [2]浙江海洋开发研究院,舟山316000 [3]温州出入境检验检疫局,温州325000

出　　处：《分析试验室》2019年第2期141-146,共6页Chinese Journal of Analysis Laboratory

基　　金：国家科技支撑计划(2012BAD29B06);舟山市社会公益科技计划(2015C31049)资助

摘　　要：建立了顶空进样-气相色谱串联三重四级杆质谱(HS-GC-MS/MS)法检测海产品中的7种苯系物。海产品经过90℃温育和振荡平衡40 min顶空进样,HP-INNOWax色谱柱分离,程序升温,在多反应监测(MRM)模式下GC-MS/MS检测,外标法定量。结果表明,7种苯系物在3. 0～100. 0μg/kg的浓度范围内呈线性,相关系数均在0. 99以上,方法检出限为1. 5～2. 5μg/kg,定量限为3. 0～5. 0μg/kg,平均回收率在82. 7%～114. 4%之间,相对标准偏差(RSD)小于9. 9%。用建立的方法测定了东海海域不同海产品中7种苯系物的含量。该法适合海产品中苯系物残留量的测定。A method based on gas chromatography-triple quadrupole mass spectrometry (HS-GC-MS/ MS)with headspace was developed for the determination of 7 benzene series in seafood.The samples were incubated at 90℃ and oscillated for 40 min,then identification and quantification of the concentrate was carried out in the multiple reaction monitoring (MRM)mode after GC separation on a HP-INNOWax column by headspace,using external standard method.The target compounds were detected in a linear dynamic range of 3.0 -100.0μg/kg with a linear determination coefficient (r^2) greater than 0.99.LOD and LOQ were 1.5 -2.5μg/kg and 3.0 -5.0μg/kg,respectively.The recoveries were in the range of 82.7%-114.4%and the relative standard deviations (RSD)were below 9.9%.The method was suitable for the determination of 7 benzene series in seafood.

关 键 词：顶空进样 GC-MS/MS 苯系物 海产品 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程] O657.7[轻工技术与工程—食品科学与工程]