稀酸加热振荡提取-电感耦合等离子质谱仪测定植物油中痕量硒  

Determination of trace selenium in vegetable oil by inductively coupled plasma mass spectrometry with dilute acid heating oscillation extraction

作　　者：倪张林[1] 袁新跃 屈明华[1] 叶彩芬[1] 汤富彬[1] NI Zhang-lin;YUAN Xin-yue;QU Ming-hua;YE Cai-fen;TANG Fu-bin(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400;The Food Safety Inspection Center of FuYang District in Hangzhou,Hangzhou 311400)

机构地区：[1]中国林业科学研究院亚热带林业研究所国家林业局经济林产品质量检验检测中心(杭州),杭州311400 [2]杭州市富阳区食品安全检验检测中心,杭州311400

出　　处：《分析试验室》2019年第2期207-211,共5页Chinese Journal of Analysis Laboratory

基　　金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2017QC002);杭州市富阳区科技计划(20171226Y188)资助

摘　　要：建立了稀酸加热振荡提取-电感耦合等离子体质谱仪测定植物油中的痕量硒方法,优化了提取过程中的温度和稀酸浓度。优化后的条件为:2 g植物加入10%(V/V)硝酸,在80℃加热振荡70 min可将植物油中的蛋白态硒转化为游离态硒并提取完全。方法线性范围为0. 2～40μg/L,方法检出限为0. 2μg/kg,加标回收率在84. 4%～105. 0%之间,相对标准偏差(RSD,n=6)小于5. 0%。该方法与传统的微波消解法测定的结果无显著性差异(P> 0. 05)。A new sample preparation method was developed to analyze the selenium in vegetable oii by inductive coupled plasma mass.Two gram oil sample was extracted by 10%(V/V)HNO3 at 80℃ water bath heating with mechanical shaking.Protein -Se could be transferred to inorganic Se,and extracted by 10%(V/V)HNO3 in 70 minutes.The linearity ranged from 0.2 to 40μg/L with the limit of detection of 0.2 μg/kg.The recoveries ranged from 84.4% to 105.0% with relative standard deviation less than 5.0% There was no significant difference (P >0.05 )of the results obtained by this method and microwave digestion method,which meant the developed method had good accuracy.

关 键 词：植物油 硒 提取法 电感耦合等离子质谱仪 

分 类 号：TS207.3[轻工技术与工程—食品科学]