萎凋对茶鲜叶不同叶位叶绿素荧光特征参数的影响  

Effects of withering on chlorophyll fluorescence characteristics of different leaf positions in fresh tea leaves

作　　者：许思敏[1] 王威[1] 王蔚[1] 王赞[1] 易国春 郭雅玲[1] XU Si-Min;WANG Wei;WANG Wei;WANG Zan;YI Guo-Chun;GUO Ya-Ling(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学园艺学院,福州350002

出　　处：《食品安全质量检测学报》2019年第3期693-699,共7页Journal of Food Safety and Quality

基　　金：乌龙茶加工体系技术创新及茶类创衍应用研究(CXZX2017350);福建农林大学科技创新专项基金项目(CXZX2016102)~~

摘　　要：目的研究萎凋对茶叶水分和叶绿素荧光参数的影响。方法采用叶绿素荧光仪Imaging PAM对福云6号7个萎凋时间点进行水分含量测定及叶绿素荧光测定。结果不同叶位叶片萎凋过程的水分含量与叶绿素荧光参数之间具有一定的内在联系。不同叶位之间的叶绿素荧光参数在萎凋后期呈显著差异;叶片水分含量与叶绿素荧光参数的初始荧光F_0、非光化学淬灭系数(non-photochemical quenching, NPQ)之间呈极显著负相关,与叶绿素荧光参数的最大光化学效率F_v/F_m、光合潜在活性F_v/F_0、电子传递效率(electrontransferrate,ETR)、实际光合量子产量Y(Ⅱ)之间呈极显著正相关。结论鉴于上述叶绿素荧光参数与叶片水分含量之间的相关性,初步认为F_0、NPQ、F_v/F_m、F_v/F_0、ETR和Y(Ⅱ)作为茶树鲜叶萎凋过程中水分胁迫程度的指标是可行的,对鲜叶萎凋过程的在线判断具有一定的意义。Objective To study the effects of withering on tea moisture and chlorophyll fluorescence parameters. Methods The chlorophyll fluorescence instrument Imaging PAM was used to determine the moisture content and chlorophyll fluorescence of the 7 withering time points of Fuyun no. 6. Results There was an internal relationship between the water content and chlorophyll fluorescence parameters of the leaf wilting process in different leaf positions. Chlorophyll fluorescence parameters in different leaf positions showed significantly different at the later stage of withering. There was a significant negative correlation between leaf water content and initial fluorescence F0 and non-photochemical quenching(NPQ) of chlorophyll fluorescence parameters. And there was a significant positive correlation between leaf moisture content and chlorophyll fluorescence parameters, maximum photochemical efficiency Fv/Fm, photosynthetic potential activity Fv/F0, electron transfer rate(ETR), and actual photosynthetic quantum yield Y(Ⅱ). Conclusion In view of the correlation between chlorophyll fluorescence parameters mentioned above and leaf moisture content, it is preliminarily considered that F0, NPQ, Fv/Fm, Fv/F0, ETR and Y(Ⅱ) are feasible indicators of water stress degree during the withering process of fresh leaves of tea trees, which has certain significance for online judgment of the withering process of fresh leaves.

关 键 词：叶绿素荧光 萎凋 水分含量 光合机构 生理活性 

分 类 号：O657.3[理学—分析化学] TS272.7[理学—化学]