不同来源香菇双单杂交后代的遗传研究  

Genetic study on hybrids from di-mon mating of different origins in Lentinula edodes

作　　者：叶明[1] 叶生梅[1] 潘迎捷 马桂荣[3] 陈永萱[4] 

机构地区：[1]安徽工程科技学院生物化工系,安徽芜湖241000 [2]食用菌遗传育种农业部重点开放实验室,上海201106 [3]山东大学微生物技术国家重点实验室,山东济南250100 [4]南京农业大学微生物学系,江苏南京210095

出　　处：《南京农业大学学报》2002年第1期53-56,共4页Journal of Nanjing Agricultural University

基　　金：安徽省教育厅科研基金资助 (2 0 0 0J10 2 9) ;安徽省自然科学基金 (0 10 41112 )资助

摘　　要：采用交配基因型遗传标记的香菇野生株Q与栽培株苏香的孢子单核体和原生质体单核体进行完全亲和双 单杂交 ,并对杂交后代进行了遗传分析。显著性检验表明 ,供试菌株的菌丝生长速率及CMC酶活力均达到极显著差异水平。RAPD结果分析表明 ,6个杂交菌株在DNA水平上具有很高的相似性 ,但来源于原生质体单核体的杂交后代与来源于孢子单核体的杂交后代存在不同程度的差异。酯酶同工酶结果显示 ,来自孢子单核体的杂交后代比来自原生质体杂交后代的变异程度高。两种不同来源的杂交后代在农艺性状方面具有不同的变异范围。Di mon mating a wild strain Q and sporualated monokaryons (SM) and protoplasted monokaryons(PM)of Suxiang derived from a cultured strain of Lentinula edodes were carried out and their hybrids were analysed.RAPD analysis indicated significant DNA similarity among six hybrids but showed a varying difference between ones from SM and hybrids from PM.F test displayed that the strains tested had a marked difference in the growth rate of mycelium and CMC enzyme activity.Hybirds of SM had higher variation than that of PM in esterase isozyme,and variation range of hybirds from SM was different from that from PM in agronomic characteristics.

关 键 词：香菇 双-单杂交 随机扩增多态性 DNA 酯酶同工酶 聚类分析 农艺性状 遗传研究 

分 类 号：S646.12[农业科学—蔬菜学]