鲨鱼软骨提取物对小鼠Lewis肺癌生长的抑制效应  

Inhibitory effect of shark cartilage extract on the growth of Lewis lung carcinoma transplanted in mice

作　　者：王曦[1] 沈先荣[1] 贾福星[1] 蒋定文[1] 任大明[2] 吉冬梅[2] 王玲[1] 储智勇[1] 雷呈祥[1] 

机构地区：[1]海军医学研究所海洋生物研究中心,上海200433 [2]复旦大学遗传所

出　　处：《中华航海医学与高气压医学杂志》2002年第1期51-54,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：上海市现代生物技术与医药发展基金 ( 9943192 18)

摘　　要：目的　探讨含有血管生成抑制因子、肿瘤细胞抑制因子和酸性粘多糖的鲨鱼软骨提取物对小鼠接种肿瘤生长的抑制效应。方法　用东海鲸鲨软骨制备得到鲨鱼软骨提取物。采用 C57BL / 6小鼠 ,小鼠 L ewis肺癌腋皮下接种模型 ,接种第 2天腹腔注射给药 ,连续给药 8d,观察抑瘤率 ;小鼠 L ewis肺癌足趾皮下接种模型 ,接种第 2天腹腔注射给药 ,连续给药 10 d,观察抑瘤率。结果　腋皮下接种模型 ,高剂量组 12 5 mg/ kg的抑瘤率为 46 .6 1% ,中剂量组 5 0 m g/ kg的抑瘤率为 37.5 6 % ,低剂量组 2 0mg/ kg的抑瘤率为 2 6 .2 4% ;足趾皮下接种模型 ,超高剂量组 2 5 0 mg/ kg抑瘤率为 5 7.6 8% ,高剂量组12 5 mg/ kg为 5 4.44 % ,中剂量组 5 0 mg/ kg为 42 .6 4% ,低剂量组 2 0 m g/ kg为 33.87%。Objective To probe the effect of shark cartilage extract on the growth of Lewis lung carcinoma in mice.Methods C 57BL/6 mice were implanted with Lewis lung carcinoma by toe or axilla subcutaneous injection.From the second day,the mice were treated daily with intraperitoneal injections of shark cartilage extract.The mice were sacrificed and autopsied at the 8th or 10th day.The inhibitory rate of tumor growth was determined by ordinary method.Results The growth of Lewis lung carcinoma was inhibited significantly by the shark cartilage extract.In the axilla model,the inhibitory rates of tumor growth were 46.61%,37.56% and 26.24% at the dose of 125 mg/kg,50 mg/kg and 20 mg/kg respectively.In the foot model,the inhibitory rates of tumor growth were 57.68%,54.44%,42.64% and 33.87% at the dose of 250 mg/kg,125 mg/kg,50 mg/kg and 20 mg/kg respectively.Conclusions The shark cartilage extract has strong inhibitory effect on the growth of Lewis lung carcinoma in mice.

关 键 词：鲨鱼软骨 LEWIS肺癌 肿瘤治疗 

分 类 号：R734.2[医药卫生—肿瘤] R931.74[医药卫生—临床医学]