有机介质中脂肪酶催化外消旋苯甘氨酸甲酯的对映体选择性氨解反应  

Lipase-catalyzed Enantioselective Ammonolysis of Racemic Phenylglycine Methyl Ester in Organic Solvent

作　　者：杜伟[1] 宗敏华[1] 郭勇[1] 何俊[1] 张媛媛[1] 谢昭霖[1] 娄文勇[1] 

机构地区：[1]华南理工大学生物工程系,广州510640

出　　处：《生物工程学报》2002年第2期242-245,共4页Chinese Journal of Biotechnology

基　　金：广东省自然科学基金资助项目 (No .980 5 43)~~

摘　　要：A novel reaction\|enzymatic ammonolysis discovered in the mid of 1990s has been demonstrated to be a very promising alternative in the preparation of optically pure compounds. The effects of organic solvent, initial water activity, temperature and additives on lipase Novozym435\|catalyzed enantioselective ammonolysis of racemic phenylglycine methyl ester were investigated systematically in this paper. Enzymatic reaction of ammonolysis showed higher activity and enantioselectivity than the corresponding reaction of hydrolysis and alcoholysis.A novel reaction\|enzymatic ammonolysis discovered in the mid of 1990s has been demonstrated to be a very promising alternative in the preparation of optically pure compounds. The effects of organic solvent, initial water activity, temperature and additives on lipase Novozym435\|catalyzed enantioselective ammonolysis of racemic phenylglycine methyl ester were investigated systematically in this paper. Enzymatic reaction of ammonolysis showed higher activity and enantioselectivity than the corresponding reaction of hydrolysis and alcoholysis.

关 键 词：脂肪酶 氨解反应 苯甘氨酸甲酯 有机介质 对映体选择性水解 抗生素中间体 

分 类 号：O621.2[理学—有机化学]