Crystalline Growth of Nitrogenase CrFe Protein  

固氮酶铬铁蛋白的晶体生长(英文)

作　　者：张华峰[1] 王耀萍[2] 吕玉兵[1] 赵颖[1] 代小虎[2] 董志刚[1] 黄巨富[1] 

机构地区：[1]中国科学院植物研究所 [2]中国科学院生物物理研究所,北京100101

出　　处：《Acta Botanica Sinica》2002年第4期400-404,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金 ( 2 97710 33);国家基础研究项目 ("973"项目 0 0 1CB10 89);国家空间项目~~

摘　　要：Under a suitable condition of crystallization, dark brown rhombohedron crystals (the lengths of the longest two diagonals were 0.25 and 0.12 mm, respectively) could be obtained from nitrogenase CrFe protein purified from a mutant UW3 of Azotobacter vinelandii Lipmann grown in Cr-containing but NH3-free Medium, The possibility of crystallization, as well as the. number, size and quality of crystals obviously depended on the concentrations of PEG 8000, MgCl2, NaCl, Tris and Hepes buffer, and methods of crystallization. The optimum concentrations of the chemicals for crystallization of CrFe protein were slightly different from those for crystallization of MnFe protein from UW3 grown in Mn and DeltanifZ MoFe protein from a nifZ deleted strain of A. vinelandii. The crystal seemed to be formed from CrFe protein.在合适的结晶条件下 ,从含Cr无氨培养基中生长的固氮菌 (AzotobactervinelandiiLipmann)突变种UW3 中纯化出的CrFe蛋白可从溶液中析出深棕色斜四棱柱晶体 ,晶体最大的两条对角线长度分别可达 0 .2 5mm和 0 .12mm。PEG 80 0 0、MgCl2 、NaCl、Tris和Hepes缓冲液的浓度及结晶方法等对该蛋白的出晶率、晶核数目、晶体大小和质量都有明显影响。CrFe蛋白结晶所需的上述化合物的最适浓度与在Mn中生长的固氮菌突变种UW3 的MnFe蛋白和缺失nifZ固氮菌突变种的ΔnifZMoFe蛋白结晶所需的最适浓度有所不同。结果表明 。

关 键 词：mutant UW3 of Azotobacter vinelandii Cr-containing medium nitrogenase CrFe protein crystalline growth 

分 类 号：Q591.2[生物学—生物化学]