云南高校图书馆联盟文献共享服务平台- 董志刚

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Crystalline Growth of Nitrogenase CrFe Protein: 《Acta Botanica Sinica》2002年第4期400-404,共5页张华峰王耀萍吕玉兵赵颖代小虎董志刚黄巨富; 国家自然科学基金 ( 2 97710 33);国家基础研究项目 ("973"项目 0 0 1CB10 89);国家空间项目~~; Under a suitable condition of crystallization, dark brown rhombohedron crystals (the lengths of the longest two diagonals were 0.25 and 0.12 mm, respectively) could be obtained from nitrogenase CrFe protein purified f...; 关键词：mutant UW3 of Azotobacter vinelandii Cr-containing medium nitrogenase CrFe protein crystalline growth

Purification and Characterization of Cr-containing Nitrogenase Component Ⅰ: 《Acta Botanica Sinica》2002年第3期297-300,共4页黄巨富董志刚张华峰吕玉兵赵颖汪志平; 国家自然科学基金 ( 2 97710 33,0 0 1CB10 89)~~; A mutant UW 3, which is unable to fix N 2 in the presence of Mo (Nif -) but can undergo phenotypic reversal to Nif + under Mo_deficient conditions, was able to grow in Cr_containing but Mo_ and NH 3_deficient...; 关键词：NITROGENASE Cr_containing protein mutant UW 3

Purification and Characteristics of Mn-containing Nitrogenase Component Ⅰ: 《Acta Botanica Sinica》2001年第9期918-922,共5页黄巨富汪道涌董志刚汪志平张华峰吕玉兵许祥明赵颖; SupportedbytheNationalNaturalScienceFoundationofChina ( 2 97710 33); A mutant UW 3, which is unable to fix N 2 in the presence of Mo (Nif -) but undergo phenotypic reversal to Nif + under Mo deficiency, was able to grow in Mo- and NH 3-deficient medium containing Mn, and the growt...; 关键词：protein purification protein characteristic nitrogenase protein containing Mn UW 3 mutant of Azotobacter vinelandii

Growth of the Crystals of Nitrogenase MnFe Protein: 《Acta Botanica Sinica》2001年第4期375-379,共5页黄巨富董志刚汪道涌吕玉兵张华峰王耀萍韩毅代小虎; SupportedbytheNationalNaturalScienceFoundationofChina (2 97710 33)andtheNationalSpaceProjectofChina .; Under a suitable condition of crystallization, dark brown short rhombohedron crystals could be obtained from nitrogenase MnFe protein purified from a mutant UW3 of Azotobacter vinelandii Lipmann grown in Mn-containing...; 关键词：mutant UW3 of Azotobacter vinelandii Mn-containing medium nitrogenase MnFe protein crystalline growth

Studies on Crystalline Growth of O_2-susceptible Proteins in Space: 《Acta Botanica Sinica》2001年第3期256-260,共5页董志刚王耀萍韩毅龚海韵汪道涌陆建华黄巨富; NationalNaturalScienceFoundationofChina (2 97710 3 3 )andtheNationalSpaceProjectofChina; In order to meet the requirement for crystalline growth of O-2-susceptible proteins in space, crystallization conditions on the earth was optimized for the proteins using a simple and suitable device for anaerobic add...; 关键词：O-2-susceptible proteins crystalline growth in space

Studies on Crystalline Growth of MoFe Protein from a nifZ Deleted Strain of Azotobacter vinelandii: 《Acta Botanica Sinica》2000年第4期383-387,共5页黄巨富王耀萍董志刚黄孝明汪道涌吕玉兵汪志平; TheNationalSpaceProjectofChina ;TheNationalNaturalScienceFoundationofChina!( 2 97710 3 3 ); Under a given condition of crystallization, dark brown short rhombohedron crystals could be obtained from Δ nifZ MoFe protein purified from a nifZ deleted mutant strain of Azotobacter vinelandii Lipmann....; 关键词：Azotobacter vinelandii nifZ deletion mutant MoFe protein crystalline growth

棕色固氮菌nifZ与固氮酶钼铁蛋白P-cluster合成的关系被引量：1: 《Acta Botanica Sinica》1999年第11期1217-1220,共4页黄巨富汪道涌董志刚汪志平李佳格王耀萍; 国家空间计划资助;国家自然科学基金; 与野生型固氮菌（OP）MoFe蛋白相比，缺失nifZ的棕色固氮菌（AzotobactervinelandiiLipann）突变种的△nifZMoFe蛋白对乙炔的还原活性和Fe／Mo比值都显著降低。在相同实验条件下从这两种MoFe蛋白中抽提出的FeMoco在催化活性和金属组成...; 关键词：棕色固氮菌 P-cluster 合成钼铁蛋白固氮酶

含铼重组液对部分缺失金属原子簇的钼铁蛋白的激活作用被引量：1: 《Acta Botanica Sinica》1999年第10期1086-1089,共4页黄巨富汪志平汪道涌董志刚李佳格; 家自然科学基金资助项目; 棕色固氮菌(AzotobactervinelandiiLipmann)固氮酶MoFe蛋白经邻菲啉和空气处理后,成为部分缺失P_cluster和FeMoco的失活蛋白。与由Re2O7、高柠檬酸铁、Na2S和二硫苏糖醇(DTT)组成的无圆二色(CD)谱信号的重组液保温后,保温蛋白对乙炔和...; 关键词：失活钼铁蛋白铼重组液体外组装含铼固氮酶

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