吕玉兵

作品数：7被引量：1H指数：1
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供职机构：中国科学院植物研究所植物分子生理学重点实验室更多>>
发文主题：NITROGENASEMUTANTGROWTHCRYSTALLINEMOF更多>>
发文领域：生物学更多>>
发文期刊：更多>>
所获基金：国家自然科学基金国家重点基础研究发展计划更多>>
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缺失nifH的棕色固氮菌突变种固氮酶钼铁蛋白的结晶
《Acta Botanica Sinica》2004年第1期58-62,共5页边少敏 赵剑峰 吕玉兵 赵颖 周会娜 王耀萍 黄巨富 
从限氨固氮培养基中培养的缺失nifH的棕色固氮菌(Azotobacter vinelandii Lipmann)突变种DJ54中，分离纯化出部分纯的缺失FeMoco的钼铁蛋白(△nifH Avl)。用相同纯化方法分别从DJ35和UW45突变种中纯化的△nifE Avl和NifB^-Avl的纯度明...
关键词：棕色固氮菌 突变种 固氮酶钼铁蛋白 NIFH FeMoco 结晶 沉淀剂 缓冲液 浓度 
Crystallization of Nitrogenase MoFe Protein from a Mutant nifE Deleted Strain of Azotobacter vinelandii被引量：1
《Acta Botanica Sinica》2003年第4期427-431,共5页赵颖 赵剑峰 吕玉兵 汪志平 王耀萍 黄巨富 
国家自然科学基金 ( 30 2 70 2 96) ;国家重点基础研究发展规化项目 ("973"项目 0 0 1CB10 89-0 6) ;国家载人航天工程项目~~
Under a suitable condition of crystallization, dark brown short rhombohedron crystals could be obtained from FeMoco-deficient MoFe protein (DeltanifE Avl) purified from a nifE deleted mutant DJ35 of Azotobacter vinela...
关键词：mutant DJ35 of Azotobacter vinelandii nitrogenase Delta nifE Av1 CRYSTALLIZATION 
Crystalline Growth of Nitrogenase CrFe Protein
《Acta Botanica Sinica》2002年第4期400-404,共5页张华峰 王耀萍 吕玉兵 赵颖 代小虎 董志刚 黄巨富 
国家自然科学基金 ( 2 97710 33);国家基础研究项目 ("973"项目 0 0 1CB10 89);国家空间项目~~
Under a suitable condition of crystallization, dark brown rhombohedron crystals (the lengths of the longest two diagonals were 0.25 and 0.12 mm, respectively) could be obtained from nitrogenase CrFe protein purified f...
关键词：mutant UW3 of Azotobacter vinelandii Cr-containing medium nitrogenase CrFe protein crystalline growth 
Purification and Characterization of Cr-containing Nitrogenase Component Ⅰ
《Acta Botanica Sinica》2002年第3期297-300,共4页黄巨富 董志刚 张华峰 吕玉兵 赵颖 汪志平 
国家自然科学基金 ( 2 97710 33,0 0 1CB10 89)~~
A mutant UW 3, which is unable to fix N 2 in the presence of Mo (Nif -) but can undergo phenotypic reversal to Nif + under Mo_deficient conditions, was able to grow in Cr_containing but Mo_ and NH 3_deficient...
关键词：NITROGENASE Cr_containing protein mutant UW  3 
Purification and Characteristics of Mn-containing Nitrogenase Component Ⅰ
《Acta Botanica Sinica》2001年第9期918-922,共5页黄巨富 汪道涌 董志刚 汪志平 张华峰 吕玉兵 许祥明 赵颖 
SupportedbytheNationalNaturalScienceFoundationofChina ( 2 97710 33)
A mutant UW 3, which is unable to fix N 2 in the presence of Mo (Nif -) but undergo phenotypic reversal to Nif + under Mo deficiency, was able to grow in Mo- and NH 3-deficient medium containing Mn, and the growt...
关键词：protein purification protein characteristic nitrogenase protein containing Mn UW  3 mutant of Azotobacter vinelandii 
Growth of the Crystals of Nitrogenase MnFe Protein
《Acta Botanica Sinica》2001年第4期375-379,共5页黄巨富 董志刚 汪道涌 吕玉兵 张华峰 王耀萍 韩毅 代小虎 
SupportedbytheNationalNaturalScienceFoundationofChina (2 97710 33)andtheNationalSpaceProjectofChina .
Under a suitable condition of crystallization, dark brown short rhombohedron crystals could be obtained from nitrogenase MnFe protein purified from a mutant UW3 of Azotobacter vinelandii Lipmann grown in Mn-containing...
关键词：mutant UW3 of Azotobacter vinelandii Mn-containing medium nitrogenase MnFe protein crystalline growth 
Studies on Crystalline Growth of MoFe Protein from a nifZ Deleted Strain of Azotobacter vinelandii
《Acta Botanica Sinica》2000年第4期383-387,共5页黄巨富 王耀萍 董志刚 黄孝明 汪道涌 吕玉兵 汪志平 
TheNationalSpaceProjectofChina ;TheNationalNaturalScienceFoundationofChina!( 2 97710 3 3 )
Under a given condition of crystallization, dark brown short rhombohedron crystals could be obtained from Δ nifZ MoFe protein purified from a nifZ deleted mutant strain of Azotobacter vinelandii Lipmann....
关键词：Azotobacter vinelandii nifZ   deletion mutant MoFe protein crystalline growth 
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