雌激素对大鼠胰腺癌前病变及K-ras基因突变影响的实验研究  

THE EXPERIMENTAL STUDY OF ESTROGEN EFFECT ON PRECANCEROUS LESION AND K-ras MUTATION IN RATS ' PANCREATIC ACINAR CELLULAR CANCER

作　　者：徐峰[1] 寿楠海[1] 姜希宏[1] 徐克森[1] 张楠[1] 

机构地区：[1]山东医科大学附属医院普外科,山东济南250012

出　　处：《中国现代普通外科进展》1999年第1期36-39,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的 建立大鼠胰腺腺泡细胞癌癌前病变的动物模型,了解雌激素对该癌前病变及K-ras基因突变的影响.方法 雄性Wistar大鼠单次腹腔注射重氮乙酰丝氨酸30mg/kg,部分大鼠2个月后开始肌肉汪射戊酸雌二醇0.18mg/100g×2周,4个月后,常规取胰腺组织行光镜和电镜检查,并应用PCR-SSCP法检测K-ras基因突变情况.结果 单纯给予重氮乙酰丝氨酸的大鼠胰腺腺泡细胞癌癌前病变发生率100%,K-ras基因突变率42.1%;经雌激素处理的大鼠胰腺腺泡细胞癌癌前病变发生率92.4%,K-ras基因突变率25.6%.结论 重氮乙酰丝氨酸可成功诱导大鼠胰腺腺泡细胞癌癌前病变,K-ras基因突变在此过程中属早期事件.雌激素一定程度上可抑制该癌癌前病变的发生发展.Objective: To set up the animal model of precancerous lesion of rats' pancreatic acinar cellular cancer,and to learn estrogen's effect on this lesion's biological feature and K-ras gene mutation. Methods: male Wistar rats were injected Azaser-ine 30mg/kg(ip). Some rats received Estradiol-17 P Valerate(0.18mg/100g/2weeks, im) .4 months later, rats'pancreas were autopsied and K-ras gene mutation were detected by PCR-SSCP method. Results : The incidence of AACN were very high. The incidence of K-ras gene mutation was 42.1% in rats without estrogen and 25.6% with estrogen. Conclusion: Azaserine could induce precancerous lesion of rats' pancreatic acinar cancer successfully, K-ras gene mutation was early change during this process Estrogen could inhibit this precancerous lesion's happen and development to some degree.

关 键 词：重氮乙酰丝氨酸 胰腺肿瘤 雌激素 K-RAS 基因突变 胰腺癌 动物实验 

分 类 号：R735.9[医药卫生—肿瘤]