充血性心衰大鼠严格限钠后肾素-血管紧张素系统的变化  

Changes of renin-angiotensin system of rats with congestive heart failure after restriction of sodium intake

作　　者：张素珍[1] 徐永[2] 万瑜[3] 杨钢[3] 

机构地区：[1]广东医学院病理生理学教研室,广东湛江524023 [2]广东医学院生理学教研室,广东湛江524023 [3]武汉大学医学院生理学教研室,湖北武汉430071

出　　处：《第四军医大学学报》2002年第6期530-532,共3页Journal of the Fourth Military Medical University

基　　金：湖北省教委青年科研基金资助项目 ( 96 C0 5 )

摘　　要：目的　观察充血性心衰大鼠严格限钠后循环与局部肾素 -血管紧张素系统 (RAS)的变化 .方法　将充血性心衰大鼠随机分为 3组 :心衰组、心衰限钠组、心衰补钠组 ,假手术大鼠为对照组 ,用放射免疫分析法和原位杂交技术分别测定各组血浆和心肌血管紧张素 (Ang ) ,醛固酮 (Ald)含量及心肌血管紧张素原 m RNA表达水平 .结果　心衰限钠组血浆 Ang ,Ald含量 [(2 2 5± 2 0 ) ng· L- 1 和 (4 76± 6 2 )μg·L- 1 ]及心肌 Ang 含量 [心房 (2 0 .1± 4.5 ) ng· g- 1 ,心室(2 7.3± 5 .9) ng· g- 1 ]显著高于心衰组 [(180± 2 9) ng· L- 1 ,(2 48± 5 8) μg· L- 1 及 (17.5± 3.6 ) ng· g- 1 ,(2 0 .1± 3.7)ng· g- 1 ](P<0 .0 5 ,P<0 .0 1) ,心室肌 Ang原 m RNA表达水平 (12 .6± 2 .3)也显著高于心衰组 (8.6± 1.7) (P<0 .0 5 ) ,血钠较心衰组下降 5 % (P<0 .0 5 ) ;心衰补钠组心肌血管紧张素原 m RNA表达水平与心衰组无显著差别 ,其余指标与对照组无显著差别 .结论　心衰后限钠进一步激活循环和局部RAS。AIM To observe Changes of circular and local renin-angiotensin system (RAS) of rats with restriction of sodium intake after congestive heart failure. METHODS Congestive heart failure (CHF) rats were divided into three groups with sham operation rats group as control. Radioimmunassay and in situ hybrdization techniques were used to determine the plasma and myocardium angiotension Ⅱ (AngⅡ) and aldosterone (Ald) contents and the expression of angiotensionogen mRNA ,respectively. RESULTS In sodium restricted group, the plasma AngⅡ and Ald contents [(225±20) ng·L -1, (476±62) μg·L -1] and myocardium AngⅡ contents [atrial (20.1±4.5) ng·g -1, ventricle (27.3±5.9) ng·g -1] were significantly higher than those in CHF group [(180±29) ng·L -1, (248±58) μg·L -1 and (17.5±3.6) ng·g -1,(20.1±3.7)ng·g -1](P<0.05, P<0.01), and the ventricle angiotensionogen mRNA expression (12.6±2.3) were also obviously higher than those in CHF group (8.6±1.7)(P<0.05), and while the plasma sodium was 5% lower than that in CHF group (P<0.05); In sodium supplemented group, the myocardium angiotensionogen mRNA expression had no obvious change compared with the CHF group, the other items had no differentiation as compared with the control. CONCLUSION Restriction of sodium intake after CHF can activate the circular and local RAS further and then worsen the sodium and water retention.

关 键 词：大鼠 充血性心力衰竭 肾素-血管紧张素系统 膳食 

分 类 号：R541.61[医药卫生—心血管疾病]