低聚壳聚糖及其金属配合物的抗O_2^(·-)活性研究  

O_2^(·-) Scavenging Activity of Chitosan and Its Metal Complexes

作　　者：尹学琼[1] 林强[2] 张岐[2] 杨丽春[2] 

机构地区：[1]海南大学理工学院化工系,海口,570228,昆明理工大学国土与资源管理工程学院,昆明 [2]海南大学理工学院化工系,海口,570228

出　　处：《应用化学》2002年第4期325-328,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金 (2 0 0 610 0 1);海南省首届高校优秀中青年教师教学与科研奖励基金资助项目

摘　　要：以 NBT/VB2 /蛋氨酸为 O· - 2 产生、检测体系 ,对自制低聚壳聚糖及其金属配合物进行了抗 O· - 2 活性研究 ,结果显示低聚壳聚糖及其金属配合物对 O· - 2 均具有明显的清除活性 .质量浓度为 0 .5× 1 0 - 2 g/m L时 ,壳聚糖对 O· - 2 的清除率达 80 .3 % ,其与氯化镧、醋酸铜、醋酸钴的配合物对 O· - 2 的清除率分别为98.9%、84.1 %、78.4% ;相同条件下 ,高分子壳聚糖和单糖对 O· - 2 没有明显的清除作用 ,清除率仅为 1 3 %、9.5 % .随着样品质量浓度降低 。The O ·- 2 scavenging activity of low molecular weight chitosan(LMC) and its complexes with Cu(Ⅱ), Co(Ⅱ), La(Ⅲ) was investigated. Colorimetric method was used, where V B2 and Met were used as producing reagent and NBT as detecting reagent for O ·- 2. The results showed that when mass concentration of samples was 0 5×10 -2 g/mL, the O ·- 2 scavenging activity of LMC and LMC Cu(Ⅱ), LMC Co(Ⅱ), LMC La(Ⅲ) were 80 3%, 98 9%, 84 1% and 78 4% respectively, but those of high molecular weight chitosan(820 kD) and D Glucosamine were only about 13% and 9 5%. The scavenging activity of LMC and its complexes decreased with decrease in their mass concentration .

关 键 词：低聚壳聚糖 金属配合物 活性氧 抗氧化活性 自由基清除剂 

分 类 号：R362[医药卫生—病理学] O636.1[医药卫生—基础医学]