以胰岛素样生长因子受体1基因为靶的反义寡核苷酸体内外抗肿瘤研究  

Antitumor Activity of Antisense Oligonucleotides Targeted to IGF1R in vivo and In vitro

作　　者：林莉[1] 王升启[2] 管伟[2] 杨秉呼[2] 胡小电[1] 

机构地区：[1]军事医学科学院附属医院,北京100039 [2]军事医学科学院放射医学研究所,北京100850

出　　处：《生物化学与生物物理进展》2002年第2期247-251,共5页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展规划项目 (G19980 5 1118);国家高技术"86 3"计划资助项目 ( 10 2 0 8 0 4 0 1)~~

摘　　要：以胰岛素样生长因子受体 - 1基因为靶筛选抗肿瘤药物 .根据IGF1RmRNA的二级结构设计了 9条反义寡核苷酸药物 ,以脂质体介导进行转染 ,MTT染色计算细胞生长抑制率 ,从中筛选出一条序列并对之进行优化 ,最后以最佳序列进行体外作用持续时间及体内细胞生长抑制率分析 .结果表明该序列在体内外具有良好的抗肿瘤活性 ,具有剂量依赖性关系 ,且对荷瘤裸鼠无明显的毒性 .In order to Screening antitumor drugs tartgeted to IGF1R gene, 9 different 20-mer anti-sensitive oligo-deoxyribonucleic acid (ASODN) were designed according to the mRNA second structure of IGF1R gene and were transfected into tumor cells under various conditions in the presence of lipofectin. Cell growth activity were evaluated by MTT assay. The best sequence with antitumor activity in vitro and in vivo were analyzed. This sequence showed strong anticancer activity in vitro and in vivo and had dose -dependent relation. The sequence had no obvious toxicity on tumor -burdended nude either. IGF1R could be used as an appropriate target for tumor therapay.

关 键 词：胰岛素样生长因子受体1 靶标 反义寡核苷酸 抗肿瘤 IGF1R 

分 类 号：R730.5[医药卫生—肿瘤] R979.1[医药卫生—临床医学]