钙通道活性与胃泌素促人结肠癌细胞增殖间关系的研究  

The relationship between the calcium channel activity and gastrininduced proliferation of HT29 colon carcinoma cells

作　　者：吴浩[1] 张正[1] 陈斌[1] 魏于全[2] 雷松[2] 

机构地区：[1]华西医科大学附属第一医院消化科,成都610041 [2]华西医科大学附属第一医院肿瘤生物治疗中心,成都610041

出　　处：《重庆医学》2002年第4期271-273,共3页Chongqing medicine

摘　　要：目的　探讨钙通道活性在胃泌素促人结肠癌细胞增殖中的作用。方法　运用流式细胞仪检测细胞内游离钙离子水平 ,MTT比色法测定细胞增殖情况。结果　 2 5× 10 -6M的五肽胃泌素 (PG)可以引起HT2 9细胞内游离钙离子水平明显升高 (P <0 0 1) ,钙通道阻滞剂nifedipine(10 -5M)可以使PG引起的钙离子水平升高幅度明显下降 (P <0 0 1)。同时可以部分抑制PG促增殖作用 (P <0 0 5 )。钙通道激动剂Bayk86 4 4 (10 -6M)可以明显升高细胞内钙离子水平 (P <0 0 1)。并能促进HT2 9细胞增殖 (P <0 0 5 ) ,与PG的促增殖作用相比无显著性差异 (P >0 0 5 )。结论　钙通道作为调节细胞内钙离子变化的主要途径 ,在调控PG促HT2Objective To study the role of calcium channel activity in regulation of pentagastrin induced proliferation of HT29 colon carcinoma cells.Methods Flow cytometry was used to monitor the cytoplasmic free calcium,MTT colorimetry was used to determine the proliferation of HT29 cell line.Results 2.5×10 -6 M pentagastrin(PG) can induced a quick rise of intracellular free calcium([Ca 2+ ] i)in HT29 cells. Calcium channel antagonist nifedipine(10 -5 M) can significantly decrease the rise of [Ca 2+ ] i elicited by PG( P <0 01).In parallel, nifedipine can partly inhibit the proliferation of HT29 cells induced by PG( P <0 05).Calcium channel agonist Bayk8644(10 -6 M) can increase the level of [Ca 2+ ] i significantly( P <0 01).At the same time, it can induce the proliferation of HT29 cells( P <0 05).There is no difference of proliferation of HT29 cells between PG and Bayk8644( P >0 05).Conclusion The calcium channel activity was important in regulation of pentagastrin induced proliferation of HT29 cells.

关 键 词：细胞增殖 细胞内游离钙离子 结肠癌 钙通道活性 胃泌素 结肠癌细胞 

分 类 号：R735.34[医药卫生—肿瘤]