EGCG辐射保护作用的研究  

RESEARCH OF RADIOPROTECTION OF EGCG

作　　者：刘官树[1] 姚思德[1] 倪谨[2] 赵芳[2] 孟祥顺 

机构地区：[1]中国科学院上海原子核研究所辐射化学开放实验室,上海201800 [2]第二军医大学放射医学研究所,上海200433

出　　处：《辐射研究与辐射工艺学报》2002年第2期87-92,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金 (39830 0 90 )资助

摘　　要：采用MTT方法和脉冲场凝胶电泳方法 ,以人肝L0 2细胞株为研究对象 ,对没食子儿茶素没食子酸脂 (epigallocatechingallate ,EGCG)的辐射保护作用进行了研究。MTT结果表明 ,EGCG在 5— 5 0 μmol/L的浓度下 ,对细胞有明显的保护作用 ,并对细胞的修复有促进作用 ;脉冲场凝胶电泳方法结果显示 ,EGCG作用可减少辐射所致的DNA双链断裂水平。The radioprotecive effects of a major component of green tea polyphenone, (-) epigallocatechin gallate (EGCG) was examined by using the radiation-induced cell death model in Human-derived liver L02 cells, with the methods of MTT and pulsed field gel electrophoresis (PFGE). EGCG substantially suppressed the radiation-induced mortality under treatment concentration of 5-50μmol/L, where the cytotoxicity was mild. Significant suppression of DNA double strand break (DSB) was also observed with treatment of cells with 50μmol/L of EGCG. Both treatment prior to and behind irradiation was effective. It could be suggested that EGCG could either scavenge OH· or repair the damage of DNA of L02cells effectively.

关 键 词：没食子儿茶素没食子酸脂 LO2细胞 MTT方法 脉冲场凝胶电泳方法 抗氧化剂 茶多酚 绿茶 药物研究 EGCG 辐射保护 

分 类 号：R977.9[医药卫生—药品] R818.74[医药卫生—药学]