采后黄花梨(Pyrus pyrifolia Nakai.)果实中丙二烯氧合酶的生理功能  

Physiological Role of Allene Oxide Synthase in Postharvest "Huanghua" Pear Fruit(Pyrus pyrifolia Nakai.)

作　　者：窦世娟[1] 陈昆松[1] 吕均良[1] 郑金土 

机构地区：[1]浙江大学华家池校区果实分子生理学与生物技术实验室,杭州310029 [2]浙江省宁波市林业局,宁波310510

出　　处：《植物生理与分子生物学学报》2002年第2期105-110,共6页Journal Of Plant Physiology and Molecular Biology

基　　金：国家自然科学基金 (3 0 170 660 );浙江省自然科学基金(ZD0 0 0 4);杭州市科技项目资助

摘　　要：以不同成熟时期黄花梨果实为材料 ,研究果实采后成熟衰老进程中丙二烯氧合酶 (AOS)与几个成熟衰老相关因子的关系 ,探讨AOS的生理功能。结果表明 :2 0℃下不同成熟时期果实成熟衰老进程中的AOS活性变化均为峰形曲线 ,活性峰值出现在采后 10～ 12d ,先于乙烯跃变峰 2～ 4d ;果实成熟衰老各种相关因子的变化峰值出现的先后顺序依次是 :脂氧合酶(LOX)、自由基 (O- ·2 )、AOS、ACC (1 氨基环丙烷 1 羧酸 )合成酶、ACC、ACC氧化酶 ,最后为乙烯跃变峰的出现。 1℃下贮藏果实的AOS活性、乙烯合成和其他成熟衰老相关酶活性均受到强烈抑制 ,ACC和O- ·2 含量也较低 ,果实衰老进程被显著延缓。推测AOS是乙烯合成的上游调控因子之一。For understanding the physiological effects of allene oxide synthase (AOS) on fruit senescence, the Chinese pear( Pyrus pyrifolia Nakai. cv. 'Huang hua')fruit was used to investigated the change of AOS activity and its relationship with other senescence related factors after harvest. The peaks of AOS activity in different maturity fruits occurred on the 10th-12th day after harvest, prior to the peaks of 1 aminocyclopropane carboxylic acid (ACC) synthaseand ACC oxidase activity, ACC content, as well as the climacteric peak of ethylene production, but posterior to the peaks of lipoxygenase (LOX) activity and free radical (O -· 2) production (Figs.1-4). When the fruits were stored at 1℃, AOS activity and other senescence related factors studied in this paper were markedly inhibited while the fruit senescence was delayed as well (Figs.1-4). It suggests that AOS is one of the up stream regulators in ethylene biosynthesis.

关 键 词：采后 黄花梨 果实 丙二烯氧合酶 生理功能 成熟衰老 AOS LOX 乙烯 

分 类 号：Q945[生物学—植物学]