牛磺酸对大鼠自由基的清除作用及其对亚硒酸钠性白内障脂质过氧化的影响  

The role of taurine as hydroxyl radical inhibitor and its effect on lipid peroxidation in selenite cataract

作　　者：张伟[1] 陈翠真[1] 董冰[1] 陆道慧[2] 陈德文[2] 

机构地区：[1]北京市眼科研究所,100730 [2]中国科学院化学所分子动态及稳态结构国家实验室

出　　处：《中华眼科杂志》2002年第3期157-160,共4页Chinese Journal of Ophthalmology

基　　金：分子动态及稳态结构国家实验室开放基金资助项目(NO97 12 )

摘　　要：目的　探讨牛磺酸对体外反应系统中的羟自由基和超氧阴离子自由基的清除作用 ;观察腹腔注射牛磺酸对大鼠亚硒酸钠性白内障晶状体中脂质过氧化的影响。方法　应用电子自旋顺磁共振技术 ,检测不同浓度的牛磺酸对羟自由基和超氧阴离子自由基的清除作用。在注射亚硒酸钠后第 2、5、9和 14天 ,分别测定正常组、亚硒酸钠组和牛磺酸组大鼠晶状体中的丙二醛 (malondialdehyde,MDA)含量。结果　牛磺酸对羟自由基和超氧阴离子自由基具有明显的清除作用。大鼠注射亚硒酸钠后 ,其晶状体中MDA的含量较正常晶状体明显升高 ,并随着白内障病程的发展逐渐增加。牛磺酸组晶状体中MDA的含量始终低于亚硒酸钠组。结论　牛磺酸对羟自由基、超氧阴离子自由基具有明显的清除作用 ;对亚硒酸钠性白内障晶状体中的脂质过氧化反应具有明显的抑制作用。Objective To study the role of taurine as hydroxyl radical and superoxide anion radical inhibitor in vitro and evaluate the effect of intraperitoneal injection of taurine on peroxidation in rat selenite cateractous lenses Methods Electron spin resonance (ESR) was used to detect hydroxyl radical and superoxide anion radical The concentrations of malondialdehyde (MDA) in normal lenses, selenite injected lenses and taurine treated lenses were measured on the 2 nd, 5 th, 9 th and 14 th days after the first injection of selenite Results Taurine was an inibitor of hydroxyl radical and superoxide anion radical Its effect was very obvious The concentration of MDA in selenite injected lenses increased significantly after injection of selenite and became higher with the development of cataract The concentration of MDA in taurine treated lenses was lower than that in selenite injected lenses Conclusion Taurine is an inhibitor of hydroxyl radical and superoxide radical It can prevent lipid peroxidation in selenite cataractous lenses

关 键 词：白内障 牛磺酸 自由基 丙二醛 亚硒酸钠性白内障 脂质过氧化 

分 类 号：R776[医药卫生—眼科]